AZDye 555 DBCO

AZDye™ 555 DBCO is a water-soluble, bright orange-fluorescent dye with excitation ideally suited for the 532 nm or 555 nm laser lines and visualized with TRITC (tetramethylrhodamine) filter sets. AZDye™ 555 conjugates of antibodies, peptides, and proteins are pH insensitive from pH 4 to pH 10. The brightness and photostability of this dye are best suited to direct imaging of low-abundance targets.

AZDye™ 555 DBCO is a bright, far-red-fluorescent, probe routinely used for imaging of azide-containing biomolecules without the need for copper catalyst. AZDye™ 555 DBCO reacts with azides via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. In application where the presence of copper is a concern AZDye™ 555 DBCO is an ideal alternative to copper requiring fluorescent alkynes.

AZDye™ 555 is structurally similar to Alexa Fluor® 555, and spectrally is almost identical to Cy3 Dye, Alexa Fluor® 555, CF® 555 Dye, or any other Cyanine3 based fluorescent dyes. AZDye™ 555 DBCO can be used as an alternative to Alexa Fluor® 555 sDIBO.