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AZDye 488 Hydroxylamine (FP-1016)

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Description

AZDye 488 Hydroxylamine (aminooxy) reacts with aldehydes and ketones to yield oximes. Oximes are superior to hydrazones with respect to hydrolytic stability. AZDye 488 Hydroxlamine often used as membrane-impermeant, aldehyde-fixable cell tracers as well as reactive dye for labeling aldehydes or ketones in polysaccharides or glycoproteins.

AZDye™ 488 (Alexa Fluor® 488 equivalent) is a bright, highly photostable, green-fluorescent probe optimally excited by the 488 nm laser line. This probe is water-soluble and its fluorescence is pH independent over a wide pH range. The brightness and photostability of AZDye™ 488 dyes are best suited to direct imaging of low-abundance targets.

Specifications

Unit Size1 mg, 5 mg, 25 mg, 100 mg
ReactivityAldehyde, ketone
Abs/Em Maxima494/517 nm
Extinction coefficient73,000 cm-1M-1
SolubilityWater, DMSO, DMF
Spectrally similar dyesAlexa Fluor® 488, CF™ 488
Molecular weight606.58 (protonated)
Storage Conditions-20°C.
Shipping ConditionsAmbient temperature

Abs/Em Spectra

AF488

Applicable patents and legal notices are available at legal notices.

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