AZDye™ 405 NHS Ester is a water-soluble, blue-fluorescent dye that is often used in multi-color applications, including flow cytometry and super-resolution microscopy using STORM. Its excitation is ideally suited for the 407 nm spectral line of the krypton laser or the 408 nm violet laser diode. AZDye™ 405 conjugates are pH-insensitive from pH 4 to pH 10.
The NHS ester (or succinimidyl ester) is the most popular amine reactive group for labeling with the primary amines of proteins (Lys), amine-modified oligonucleotides, and other amine-containing molecules. Proteins can be labeled with AZDye™ 405 NHS Ester at high molar ratios without significant self-quenching, leading to brighter conjugates and more sensitive detection.
| Unit Size | 1 mg, 5 mg, 25 mg, 100 mg |
|---|---|
| Reactivity | Primary amines |
| Abs/Em Maxima | 402/424 nm |
| Extinction coefficient | 33,000 cm-1M-1 |
| Solubility | Water, DMSO, DMF |
| Spectrally similar dyes | Alexa Fluor® 405, DyLight® 405, Cascade Blue |
| Molecular weight | 726.05 (protonated) |
| Storage Conditions | -20°C. |
| Shipping Conditions | Ambient temperature |
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