Streptavidin is a 60 kDa non-glycosylated protein composed of four identical subunits, each of which has a binding site for biotin. Our streptavidin is isolated from Streptomyces avidinii by a specific affinity system which results in a homogeneous, ultrapure streptavidin with very low nonspecific binding characteristics.
Unconjugated streptavidin is recommended for use in solid-phase assays and immunohistochemical applications when the technique of sequential addition of reagents (sandwich technique) is employed. This reagent has also been effectively employed in subtractive hybridization procedures.
Features:
Resembles egg white avidin in its biotin binding properties
In most applications streptavidin and avidin are interchangeable
Recommended method of reconstitution: dissolve in 10 mM phosphate,
0.15 M NaCl, pH 7.5. Reconstituted solutions of streptavidin may be
preserved with 0.08% sodium azide.
The recommended concentration range for use is 5-20 µg/ml.