The VECTASTAIN ABC-AmP is an amplified ABC alkaline phosphatase reagent for the detection of mouse or rabbit primary antibodies on nitrocellulose or PVDF membranes. The signal can be visualized using either a chemiluminescent / chemifluorescent or a chromogenic substrate. Used with the DuoLuX Chemiluminescent / Chemifluorescent Substrate, the VECTASTAIN ABC- AmP system produces a very high and sustained light emission signal, with low background, and permanent fluorescence.
Uses biotin/streptavidin technology
Can detect as little as 1 pg target protein when used with DuoLuX Substrate
Standard Kit contains Reagent A and Reagent B only
Sufficient reagents to develop twenty 100 cm2 blots
Greater sensitivity can be achieved by using an amplified detection procedure. Our biotin/avidin and biotin/streptavidin systems introduce a large number of enzymes to the target site providing signal amplification while maintaining low background.