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VECTASTAIN® ABC-AmP Reagent (Standard, Western Blot Detection)

AK-6000
SKU Unit Size Price Qty
AK-6000 1 kit
$194.00
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Description

The VECTASTAIN ABC-AmP is an amplified ABC alkaline phosphatase reagent for the detection of mouse or rabbit primary antibodies on nitrocellulose or PVDF membranes. The signal can be visualized using either a chemiluminescent / chemifluorescent or a chromogenic substrate. Used with the DuoLuX Chemiluminescent / Chemifluorescent Substrate, the VECTASTAIN ABC- AmP system produces a very high and sustained light emission signal, with low background, and permanent fluorescence.

Features:

  • Uses biotin/streptavidin technology
  • Can detect as little as 1 pg target protein when used with DuoLuX Substrate
  • Standard Kit contains Reagent A and Reagent B only
  • Sufficient reagents to develop twenty 100 cm2 blots

Specifications

More Information
Unit Size 1 kit
Applications Blotting Applications, Elispot
Technology ABC (Streptavidin/Biotin) Systems
Detection Enzyme(s) Alkaline Phosphatase
Target Species No antibody included
Format Concentrate

Kit Components

Kit Contents:

  • 0.5 ml Reagent A and 0.5 ml Reagent B

Documents

Related Products

Prostate: Anti-PSA (mouse monoclonal, VP-P981), ImmPRESS™ AP Anti-Mouse Ig Reagent (MP-5402), ImmPACT™ Vector® Red Alkaline Phosphatase Substrate (SK-5105), viewed under brightfield microscopy. ImmPACT® Vector® Red Substrate Kit, Alkaline Phosphatase (AP) Catalog #: SK-5105
Colon Carcinoma: Pan-Cytokeratin (m), VECTASTAIN ABC-AP Kit, Vector Black (brown-black). Vector® Black Substrate Kit, Alkaline Phosphatase (AP) Catalog #: SK-5200
Tonsil: Cytokeratin (AE1/AE3 (m), ImmPRESS-AP Reagent, Vector Blue AP Substrate (blue). Vector® Blue Substrate Kit, Alkaline Phosphatase (AP) Catalog #: SK-5300
DuoLuX® Chemiluminescent and Fluorescent Substrate, Alkaline Phosphatase (AP) DuoLuX® Chemiluminescent and Fluorescent Substrate, Alkaline Phosphatase (AP) Catalog #: SK-6605-100
10x Casein Solution 10x Casein Solution Catalog #: SP-5020-250
Goat Anti-Rabbit IgG Antibody (H+L), Biotinylated Goat Anti-Rabbit IgG Antibody (H+L), Biotinylated Catalog #: BA-1000-1.5
Horse Anti-Mouse IgG Antibody (H+L), Biotinylated Horse Anti-Mouse IgG Antibody (H+L), Biotinylated Catalog #: BA-2000-1.5
DuoLuX® Chemiluminescent and Fluorescent Substrate, Peroxidase (HRP) DuoLuX® Chemiluminescent and Fluorescent Substrate, Peroxidase (HRP) Catalog #: SK-6604-200
Prostate: PSA (m), VECTASTAIN ABC-AP Mouse IgG Kit, BCIP/NBT (indigo), viewed under bright field microscopy. BCIP/NBT Substrate Kit, Alkaline Phosphatase (AP), (5-bromo-4-chloro-3-indolyl phosphate/nitroblue tetrazolium) Catalog #: SK-5400

Citations

Technical Information

Greater sensitivity can be achieved by using an amplified detection procedure. Our biotin/avidin and biotin/streptavidin systems introduce a large number of enzymes to the target site providing signal amplification while maintaining low background.




Home All Products VECTASTAIN ABC (Avidin-Biotin Complex Kits) VECTASTAIN ABC Kits for Western Blot Detection VECTASTAIN® ABC-AmP Reagent (Standard, Western Blot Detection)