AZDye 800Z Gly-Gly-Gly

Water-soluble, substrate for sortase mediated labeling of proteins. Sortase catalyzes a transpeptidase reaction between a specific internal sequence of a protein and an amine group present on the N-terminus of triglycine recently has become an area of great interest. This method of labeling proteins has been denoted as “Sortagging”.

AZDye 800Z is a conceptually new class of sterically shielded NIR cyanine heptamethine dye. This dye contains two shielding PEG arms directly over both faces of the heptamethine fluorochrome blocking any undesired bimolecular association processes and thus dramatically enhances the fluorescence brightness. In addition, AZDye 800Z is a zwiteroinic, charge-balanced dye with an equal number of anionic sulfonate and cationic ammonium residues, a structural feature that is known to reduce interactions with off target biological surfaces.