Your journey to deeper insights starts with Glysite™ Scout
Explore your tissue's sweet spot and home in on what matters most with a comprehensive view of glycan expression. Glysite™ Scout Glycan Screening Kits, Immunofluorescence, are fully integrated kits for the detection of glycan expression in tissue sections. Our curated lectin selection enables detection of all the major glycan motifs for the evaluation of glycan distribution in a target specimen.
Rapidly advance your research with a comprehensive view of glycan expression in your tissue sample. From biomarker discovery and validation to exploring differences in diseased and healthy tissue, these kits will help you transform your tissue insights. Ask questions that were previously beyond your reach. Start your journey of discovery and see where glyco-insights will take you.
FFPE human prostate stained with Glysite Scout Glycan Screening Kit, Immunofluorescence 488 (GSK-3000, green), showing B-GNL staining. Nuclear detail was visualized by DAPI staining (blue). Tissue was treated with Vector® TrueVIEW® Autofluorescence Quenching Kit (SP-8400). Slides were imaged at 20x.
FFPE human colon carcinoma stained with Glysite Scout Glycan Screening Kit, Immunofluorescence 594 (GSK-2000, red) showing B-ECL staining, with colocalization of the pan-cytokeratin antibody AE1/AE3 (green, primary antibody detected with a Dylight™ 488-labeled horse anti-mouse IgG secondary antibody). The tissue was treated with Antigen Unmasking Solution, Citrate-Based (H-3300) and Vector® TrueVIEW® Autofluorescence Quenching Kit (SP-8400). Nuclear detail was visualized by DAPI staining (blue). Slides were imaged at 20x.
FFPE human prostate stained with Glysite Scout Glycan Screening Kit, Immunofluorescence 649 (GSK-1000, purple), showing B-PHA-L staining. Nuclear detail was visualized by DAPI staining (blue). Tissue was treated with Vector® TrueVIEW® Autofluorescence Quenching Kit (SP-8400). Slides were imaged at 20x.
Why Glysite Scout?
Seek and you will find
Included in the kit is a diverse panel of glycan binders (lectins) ideal for the preliminary screening of tissue samples with unknown glycosylation changes with specificity to mannose, complex N-glycan, core O-glycan, fucose, sialic acid, sulfation, GlcNAc, chitin, Gal, and LacNAc. In addition to the glycan binders, the kit includes optimized immunofluorescence reagents for glycan detection, including blocking solutions, streptavidin DyLight™, and VECTASHIELD Vibrance® Antifade Mounting Media with DAPI. Validated on human and mouse tissue—including FFPE tissue, across a range of tissue types including colon, lung, spleen, kidney, liver, pancreas, testes, heart, bladder, and uterus—this versatile kit is flexible to meet your needs. Glysite Scout Glycan Screening Kits, Immunofluorescence are available with 3 fluorophore options, 488 (GSK-3000), 594 (GSK-2000), and 649 (GSK-1000).
Resources to help you get started
Lectins Application and Resource Guide
Learn how lectins are used in identifying changing glycan profiles in normal and disease states, and as valuable tools for cellular marking, neural tracing, in vivo vasculogenesis mapping, viral capture, and much more. Download now>
Discover how you can start your journey of discovery with Glysite Scout Glycan Screening Kits, Immunofluorescence. Download now>
Learn how you can save time and enjoy convenience with our streamlined workflow in our helpful how-to video.
Each of the three Glysite Scout Glycan Screening Kits, Immunofluorescence contain the same lectins but with a different fluorescent streptavidin detection reagent, offering a choice of Dylight™ 488 (GSK-3000), Dylight™ 594 (GSK-2000), or Dylight™ 649 (GSK-1000). This allows users to choose the kit which best matches the capabilities of their fluorescent microscope and also provides flexibility for multiplexing with other fluorescent detection reagents they might already be using.
Virtually any tissue type and species are suitable for use with a Glysite Scout Glycan Screening Kit, Immunofluorescence, as glycans are ubiquitous in nature and in life. The kit has been validated on human and mouse tissue—including FFPE tissue, across a range of organs, including colon, lung, spleen, kidney, liver, pancreas, testes, heart, bladder, and uterus.
If co-labeling with a primary antibody, users should optimize each protocol separately to achieve maximum signal to noise ratios. Once this is complete, the two protocols can be combined, with the Glysite Scout Glycan Screening Kit protocol applied first, followed by the antibody staining protocol. Users should perform the proper controls to demonstrate that the reagents in each protocol are not cross-reacting with each other.
DyLight™ is a trademark of Pierce Biotechnology, Inc.