VECTASHIELD® Antifade Mounting Media
Antifade mounting media optimized for immunofluorescence applications
Choosing an effective mounting medium is especially important for immunofluorescence imaging. Fluorophores are susceptible to photobleaching and fading from both the imaging excitation light and during storage. The right mounting medium will protect your samples for short- and long-term use and archiving.
The VECTASHIELD, VECTASHIELD® PLUS, VECTASHIELD Vibrance®, and VECTASHIELD HardSet™ Antifade Mounting Media are well-established, market-leading products that complete the workflow and provide excellent signal retention for image acquisition and specimen archiving.
VECTASHIELD PLUS protects against fading across the visible spectrum, even under far-red wavelengths. It is compatible with most commercially available fluorophores. This new formulation of non-setting media improves upon the original VECTASHIELD products. Specifically, VECTASHIELD PLUS exhibits no inherent background or toning and provides superior fluorophore signal retention across the spectrum, including far-red wavelengths.
VECTASHIELD Vibrance is an aqueous mountant that sets at room temperature. Slides are viewable within one hour after mounting and can be stored at room temperature for extended periods, without loss of signal intensity, retraction of media or bubble formation. VECTASHIELD Vibrance Antifade Mounting Media provides excellent protection against fading across the visible spectrum, even under far-red wavelengths. It are compatible with most commercially available fluorophores
Figure out which VECTASHIELD kit is right for you
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Venn diagram showcasing the applications for which our various VECTASHIELD antifade mounting media are recommended.
Check out this video on coverslipping tips for Immunofluorescence slide mounting
Resources
How to mount a coverslip on a slide
Correctly coverslipping your slides ensures that your tissue specimens are preserved for optimal imaging and viewing. In this blog post, we will walk you through how to properly coverslip your slides to protect your samples.
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How to choose antifade mounting media
As far as antifade mounting media go, one-size-fits-all solutions are few and far between. Here, we break down key factors you should consider when choosing the best antifade mounting medium for your experiment.
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Setting media vs non-setting mounting media: Which is right for you?
You’ve got lots of options when it comes to choosing a mounting media. Let us help you decide whether setting or non-setting mounting media will work best for your workflow in this Tips & Tricks article.
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Producing immunofluorescence (IF) staining results with high specificity and sensitivity begins with choosing the best reagents. This guide will help you:
- Learn about the IF workflow and how to optimize each step
- Quickly identify and choose the most appropriate fluorescent secondary conjugates
- Select the best anti-fade medium to preserve your fluorescence signal for imaging and archiving
Mounting, the last step in the IF workflow, is critical for tissue integrity, optical clarity, long-term storage, signal intensity, and fluorescence preservation. This IF mounting guide provides information to help researchers choose the best mounting medium for their project and offers tips to help reduce photobleaching during the image visualization process.
Product specific information for VECTASHIELD PLUS Antifade Mounting Media is available for download in this concise brochure. Application notes, key advantages, competitor comparison data, informative tables & charts.
Product specific information for VECTASHIELD Vibrance antifade mounting media is available for download in this concise brochure. Application notes, key advantages, competitor comparison data and customer testimonials are featured.
VECTASHIELD Mounting Media are well-established, market-leading products that complete the workflow and provide excellent signal retention for image acquisition and specimen archiving.
