Blocking Reagents
Reduce Noise, Enhance Clarity.
Blocking Solutions for Reliable Immunostaining Results
Blocking solutions can prevent background interference from different sources, including autofluorescence, endogenous enzyme activity, endogenous biotin, or interactions of detection reagents with tissue/cell proteins and other macromolecules. By effectively preventing nonspecific binding, these blocking reagents ensure optimal signal detection and clarity in your experiments.
Vector Laboratories offers blocking solutions for a variety of applications. Explore our comprehensive selection of high-performance blocking reagents tailored to meet your specific needs.
Protein Blocking Reagents
Protein blocking reagents prevent non-specific protein interactions between detection reagents and target protein elements that can cause unwanted background staining.
Normal Sera
A product widely used as a general protein block in tissue and cell-based staining. For the best results, choose a serum species for blocking that matches the species of the secondary antibody.
Carbo-Free Blocking Solution
A product suitable for lectin staining or use in multiplex IHC applications when lectins are used for at least one label because this solution does not contain interfering glycoproteins.
Bovine Serum Albumin (BSA)
Our BSA is free of the glycoproteins and impurities that can be present in other grades of BSA and can introduce artifacts or increase background staining. For use in IHC, ICC, IF, blotting, and ELISA applications.
Animal-Free Blocking
A plant-protein derived universal antibody diluent and protein blocking reagent for use in IHC and IF workflows, especially in multiplexing applications where primary or secondary antibodies from multiple species are used.
Endogenous Enzyme Blocking Reagents
Prevent interference from endogenous enzymes such as peroxidase or alkaline phosphatase in your assays. Vector’s enzyme blocking reagents are designed to inhibit these enzymes, ensuring clear and accurate signal development.
BLOXALL®
A dual enzyme blocking reagent that inactivates endogenous peroxidase, pseudoperoxidase, and alkaline phosphatase.
Levamisole
A competitive inhibitor of alkaline phosphatase which can be added to the alkaline phosphatase substrate solution.
Without BLOXALL
With BLOXALL
Endogenous alkaline phosphatase (AP) and peroxidase (HRP) activities in frozen intestine revealed with Vector® Red AP Substrate (red) and ImmPACT® DAB HRP Substrate (brown) (left). Same substrates used on BLOXALL Solution-treated tissue (right).
Autofluorescence Quencher
Vector TrueVIEW® Autofluorescence Quenching Kit specifically binds and quenches autofluorescent elements from non-lipofuscin sources, significantly enhancing signal-to-noise in most immunofluorescence assays.
Without TrueVIEW
With TrueVIEW
Human tonsil (FFPE); Section stained for AE1/AE3 using fluorescein label (green). Treated with TrueVIEW and mounted with VECTASHIELD® Vibrance Antifade Mounting Medium with DAPI (nuclei blue).
Species-on-Species Blocking
When an unconjugated primary antibody is made in the same species as your tissue specimen, subsequent detection using a secondary antibody usually generates high background staining and requires additional blocking.
Figure 2: LEFT image: Serial sections of human kidney (FFPE) showing strong, specific staining (brown regions) using human anti-cytokeratin primary antibody detected with HOH-3000. RIGHT image: Negative control. Serial sections of human kidney (FFPE) showing an absence of staining (no background) with the omission of the primary antibody in the HOH-3000 assay. Note: Both sections counterstained with hematoxylin (blue nuclei).
Why Choose Vector Laboratories?
High-Quality Standards
Each reagent undergoes rigorous testing to ensure top-notch performance across a range of applications.
Broad Compatibility
Our blocking reagents are designed for use in various experimental workflows, offering flexibility and reliability.
Improved Signal Clarity
By reducing background interference, our products help achieve the most accurate and reproducible results.
Expert Support
Our scientific team is here to help you choose the right reagent for your specific research needs.
Benchmarking Performance
Vector Laboratories’ reagents deliver industry-leading performance, setting the gold standard results and providing researchers with superior quality data.
Trusted and Cited in Publications
Our products are widely trusted and cited in high-impact scientific journals, highlighting their credibility and reliability in advancing groundbreaking research.
Need Help Choosing the Right Reagent?
Our team of experts is ready to assist you in selecting the most suitable blocking reagent for your specific experimental conditions.
Contact Us for personalized recommendations and technical support.
Blocking Reagent Technical and Educational Resources
To learn more about Vector’s blocking reagents for a variety of applications, explore the following resources.
Blocking Guide
This guide provides practical solutions to each potential source of non-specific staining in IHC and IF applications.
Blocking Guide
This guide provides practical solutions to each potential source of non-specific staining in IHC and IF applications.
Blocking Guide
This guide provides practical solutions to each potential source of non-specific staining in IHC and IF applications.
