Maackia Amurensis Lectin I (MAL I), Fluorescein

Maackia amurensis lectin I binds gal (β-1,4) glcNAc but tolerates substitution of N-acetyllactosamine with sialic acid at the 3 position of galactose. However, MAL I does not appear to bind this structure when substitution with sialic acid is on the 6 position of galactose.

Fluorescein labeled Maackia amurensis lectin has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes. The excitation maximum is at 495 nm and the emission maximum is at 515 nm.

$213.00

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SKU: FL-1311-2
Molecular Weight
130
Color of Fluorescence
Green
Extinction Coefficient
1.4
Formulation
10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide. 0.1 mM CaCl2
Inhibiting or Eluting Sugar
Lactose or Mucin
Maximum Emission
514-521 nm
Maximum Excitation
495-500 nm
Unit Size
2 mg
Storage Instructions
2-8°C
Sugar Specificity
Terminal 3-O sulfated Galactose on LacNAc, and terminal α2,3-sialic acid on type 2 LacNAc
Usage Summary
The recommended concentration range for use is 5-20 µg/ml.
Applications
Immunofluorescence, Glycobiology
Concentration
2 mg active conjugate/ml
Conjugate
Fluorescein
Technical Information

This lectin is the leucoagglutinin or mitogenic isolectin from Maackia seeds.

Accompanying each fluorescent lectin is an analysis data sheet summarizing the results of our quality control tests and providing pertinent information on the product. All of these reagents are supplied as solutions preserved with sodium azide.

Geisler, C and Jarvis, D.L. Glycobiology, vol. 21, no. 8 pp. 988-993, 2011

Inhibiting Sugar: 200 mM lactose