Vector® TrueVIEW® Autofluorescence Quenching Kit
Dramatically reduce autofluorescence to reveal true immunofluorescence
In tissue sections, autofluorescence is the unwanted fluorescence that can make it difficult or impossible to distinguish antigen-specific signal from non-specific background noise. The novel, patent-pending TrueVIEW Autofluorescence Quenching Kit specifically binds and quenches autofluorescent elements from non-lipofuscin sources, significantly enhancing signal-to-noise in most immunofluorescence assays—even in the most challenging tissues.
WITH TrueVIEW Quencher
Our customers have great things to say about the TrueVIEW Autofluorescence Kit!
Why TrueVIEW Quencher?
- Specific reduction of autofluorescence from non-lipofuscin sources
- Easy-to-use, one-step method
- Quick 5 min incubation
- Compatible with a wide selection of fluorophores
- Compatible with standard epifluorescence and confocal laser microscopes
Comparison with other autofluorescence reducing agents
Whereas most methods for reducing tissue autofluorescence act primarily on lipofuscin granules, the TrueVIEW quencher targets fluorescence from non-lipofuscin sources, including aldehyde fixation, red blood cells, and structural elements, such as collagen and elastin. It provides a clear, unambiguous “true view” of target antigen localization, even in problematic tissues, such as kidney, spleen, and pancreas.
Comparisons with other commercial and “home brew” approaches show that TrueVIEW is easier to use and more effective at reducing autofluorescence. The images below show the results of side-by-side comparisons on serial sections of formalin-fixed, paraffin embedded human pancreas visualized using a standard fluorescein (green) filter. No specific immunofluorescence staining was conducted.
No Treatment (Endogenous autofluorescence)
TrueVIEW Quencher Treated
Competitive treatments for dealing with autofluorescence
Alternative treatments for dealing with autofluorescence
Copper Sulfate Solution
Sudan Black B
Additional resources available from Vector Labs
Listen to the podcast
LISTEN to the podcast by Timothy Karpishin, PhD, Director of Chemistry at Vector Laboratories, Inc. describing TrueVIEW Autofluorescence Quenching Kit at the 43rd Annual NSH Symposium/Convention.
Download the poster
DOWNLOAD the poster describing TrueVIEW Autofluorescence Quenching Kit presented at the 43rd Annual NSH Symposium/Convention.
Download the brochure for more information
Learn more about the TrueVIEW Autofluorescence Quenching Kit by downloading the brochure.
The TrueVIEW Autofluorescence Kit is easy to use
Following completion of immunofluorescence staining:
Mode of Action
Product Details & Ordering Information
|Product||Catalog Number||Applications||Unit Size|
TrueVIEW Autofluorescence Quenching Kit
|SP-8400||Immunofluorescence, In situ hybridization||15 mL|
|TrueVIEW Autofluorescence Quenching Kit with DAPI||SP-8500||Immunofluorescence, In situ hybridization||15 mL|
|VECTASHIELD® Vibrance™ Antifade Mounting Medium||H-1700||Immunofluorescence, In situ hybridization, Cellular Imaging||2 mL, 10 mL|
|VECTASHIELD Vibrance with DAPI Antifade Mounting Medium||H-1800||Immunofluorescence, In situ hybridization, Cellular Imaging||2 mL, 10 mL|
Blanco, S. et al. 2020. Hyaluronate Nanoparticles as a Delivery System to Carry Neuroglobin to the Brain after Stroke. Pharmaceutics (https://doi.org/10.3390/pharmaceutics12010040)
Hou, Y. et al. 2020. SARS-CoV-2 Reverse Genetics Reveals a Variable Infection Gradient in the Respiratory Tract. Cell. 182, 1–18 (https://doi.org/10.1016/j.cell.2020.05.042)
Takenaga, K. et al. Cancer cell-derived interleukin-33 decoy receptor sST2 enhances orthotopic tumor growth in a murine pancreatic cancer model. PLoS ONE 15(4): e0232230. (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7185704/)
Fiock, K. et al. 2020. Increased Tau Expression Correlates with Neuronal Maturation in the Developing Human Cerebral Cortex. eNeuro. 7(3): ENEURO.0058-20.2020. (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7262004/)
Vinton, C.L. et al. 2019. Simian Immunodeficiency Virus Infection of Rhesus Macaques Results in Delayed Zika Virus Clearance. mBio (https://doi.org/10.1128/mBio.02790-19)
Oswald, D.M., Jones, M.B., Cobb, B.A. 2019. Modulation of hepatocyte sialylation drives spontaneous fatty liver disease and inflammation. Glycobiology (https://academic.oup.com/glycob/advance-article-abstract/doi/10.1093/glycob/cwz096/5628931)
Ushioda, W. et al. 2019. Neuropathology in Neonatal Mice After Experimental Coxsackievirus B2 Infection Using a Prototype Strain, Ohio-1. Journal of Neuropathology & Experimental Neurology (https://academic.oup.com/jnen/advance-article-abstract/doi/10.1093/jnen/nlz124/5679985)
Bencze, J., et al. 2019. Neuropathological characterization of Lemur tyrosine kinase 2 (LMTK2) in Alzheimer’s disease and neocortical Lewy body disease. Scientific Reports (https://www.nature.com/articles/s41598-019-53638-9)
Davies, S.P. et al. 2019. Hepatocytes Delete Regulatory T Cells by Enclysis, a CD4+ T Cell Engulfment Process. Cell Reports (https://www.sciencedirect.com/science/article/pii/S2211124719312653)
Liebers, J. et al. 2019. 3D image analysis reveals differences of CD30 positive cells and network formation in reactive and malignant human lymphoid tissue (classical Hodgkin Lymphoma). PLoS One (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6812863/)
Lecocq, Q. et al. 2019. Noninvasive Imaging of the Immune Checkpoint LAG-3 Using Nanobodies, from Development to Pre-Clinical Use. Biomolecules (https://www.mdpi.com/2218-273X/9/10/548/htm)
Motoike, S. et al. 2019. Clumps of Mesenchymal Stem Cell/Extracellular Matrix Complexes Generated with Xeno-Free Conditions Facilitate Bone Regeneration via Direct and Indirect Osteogenesis. International Journal of Molecular Sciences (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6720767/)
Wilson, M.R. et al. 2019. ARID1A and PI3-kinase pathway mutations in the endometrium drive epithelial transdifferentiation and collective invasion. Nature Communications (https://www.nature.com/articles/s41467-019-11403-6)
Nagai-Okatani, C. et al. 2019. Wisteria floribunda agglutinin staining for the quantitative assessment of cardiac fibrogenic activity in a mouse model of dilated cardiomyopathy. Laboratory Investigation (https://www.nature.com/articles/s41374-019-0279-9)
Sheller-Miller, S. et al. 2019. Cyclic-recombinase-reporter mouse model to determine exosome communication and function during pregnancy. American Journal of Obstetrics and Gynecology (https://www.sciencedirect.com/science/article/abs/pii/S0002937819307744#!)
Abe, H. et al. 2019. Correlation between platelet thrombus formation on collagen-coated beads and platelet aggregation induced by ADP. Transfusion and Apheresis Science (https://www.trasci.com/article/S1473-0502(19)30090-4/abstract)
Singh, B. et al. 2019. Tau is required for progressive synaptic and memory deficits in a transgenic mouse model of α-synucleinopathy. Acta Neuropathologica (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6778173/)
Chafe, S.C. et al. 2019. Targeting hypoxia-induced carbonic anhydrase IX enhances immune-checkpoint blockade locally and systemically. Cancer Immunol Res (http://cancerimmunolres.aacrjournals.org/content/early/2019/05/31/2326-6066.CIR-18-0657)
Rhodes, S. et al. 2019. Cdkn2a (Arf) loss drives NF1-associated atypical neurofibroma and malignant transformation. Human Molecular Genetics (https://academic.oup.com/hmg/article/28/16/2752/5489753)
Rodgers, H.M. et al. 2019. Dopamine D1 and D3 receptor modulators restore morphine analgesia and prevent opioid preference in a model of neuropathic pain. Neuroscience (https://www.sciencedirect.com/science/article/abs/pii/S0306452219301927)
Yoon, J.H., Li, M., Basile, J.R., Lin, Y 2018. Computer-assisted analysis of immunohistological parameters in oral giant cell granulomas. Oral Diseases (https://doi.org/10.1111/odi.13022)
Nishimura, A. et al. 2018. Hypoxia-induced interaction of filamin with Drp1 causes mitochondrial hyperfission–associated myocardial senescence. Science Signaling (https://stke.sciencemag.org/content/11/556/eaat5185.long)
Su Y, Hou Y, Wang Q. 2018. The enhanced replication of an S-intact PEDV during coinfection with an S1 NTD-del PEDV in piglets. Veterinary Microbiology (https://doi.org/10.1016/j.vetmic.2018.11.025)
Soontornniyomkij, V. et al. 2018. Association of antiretroviral therapy with brain aging changes among HIV-infected adults. AIDS (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6115290/)
Du, H. et al. 2018. A novel mouse model of hemangiopericytoma due to loss of Tsc2. Human Molecular Genetics. (https://doi.org/10.1093/hmg/ddy289)
Boucher, J.M. et al. 2018. Rab27a regulates human perivascular adipose progenitor cell differentiation. Cardiovascular Drugs and Therapy (https://doi.org/10.1007/s10557-018-6813-y)