Dramatically Reduce Autofluorescence

Vector® TrueVIEW™ Autofluorescence Quenching Kit

Reveal true immunofluorescence—even in the most challenging tissues

In tissue sections, autofluorescence is the unwanted fluorescence that can make it difficult or impossible to distinguish antigen-specific signal from non-specific background. The novel, patent-pending Vector® TrueVIEW™ Autofluorescence Quenching Kit specifically binds and quenches autofluorescent elements from non-lipofuscin sources, significantly enhancing signal-to-noise in most immunofluorescence assays.

Why TrueVIEW™ Quencher?
  • Specific reduction of autofluorescence from non-lipofuscin sources
  • Easy-to-use, one-step method
  • Quick 5 min incubation
  • Compatible with a wide selection of fluorophores
  • Compatible with standard epifluorescence and confocal laser microscopes

WITHOUT Treatment

Human Spleen not Treated with TrueVIEW™ Autofluorescence Quenching Kit

WITH Vector® TrueVIEW™ Quencher

Reduction of Autofluorescence in Human Spleen using the TrueVIEW™ Autofluorescence Quenching Kit

 

Reduction of autofluorescence in human spleen using the TrueVIEW™ Autofluorescence Quenching Kit. Human spleen sections (FFPE) stained using mouse anti-CD20 (red) and rabbit anti-Ki67 (green) primary antibodies detected with VectaFluor™ Duet kit (DK-8818). Note significant reduction of autofluorescence in the treated section.

Our customers have great things to say about the Vector® TrueVIEW Autofluorescence Kit!

Publications:

  1. Wilson, M. et al. 2020. Lgr5-positive endothelial progenitor cells occupy a tumor and injury prone niche in the kidney vasa recta. Stem Cell Research. Volume 46, July 2020, 101849. (https://www.sciencedirect.com/science/article/pii/S1873506120301501#!)

  2. Milstone, Z. et al. 2020. Histone deacetylases 1 and 2 silence cryptic transcription to promote mitochondrial function during cardiogenesis. Science Advances. Vol. 6, no. 15, eaax5150 (https://advances.sciencemag.org/content/6/15/eaax5150)

  3. Duler, L. et al. 2020. Identification of Neutrophil Extracellular Traps in Paraffin-Embedded Feline Arterial Thrombi using Immunofluorescence Microscopy. Journal of Visualized Experiments. (157), e60834, doi:10.3791/60834 (https://www.jove.com/video/60834/identification-neutrophil-extracellular-traps-paraffin-embedded?status=a62840k)

  4. Takahashi, K. et al. 2020. Eosinophils are the main cellular targets for oral gene delivery using Lactic acid bacteria. Vaccine. 38(17):3330-3338. (https://www.ncbi.nlm.nih.gov/pubmed/32197922)

  5. Parsons Aubone, A. et al. 2020. Presence of Clock genes in equine full-term placenta. Journal of Animal Science. skaa094 (https://academic.oup.com/jas/advance-article-abstract/doi/10.1093/jas/skaa094/5811452)

  6. Williams, J. et al. 2020. Mylk3 null C57BL/6N mice develop cardiomyopathy, whereas Nnt null C57BL/6J mice do not. Life Sci Alliance. 3(4): e201900593. (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7103425/)

  7. Plant, T. et al. 2020. Semaphorin 3F signaling actively retains neutrophils at sites of inflammation. The Journal of Clinical Investigation. (https://www.jci.org/articles/view/130834)

  8. de Barros Mucci, D. et al. 2020. Impact of maternal obesity on placental transcriptome and morphology associated with fetal growth restriction in mice. International Journal of Obesity. (https://www.nature.com/articles/s41366-020-0561-3)

  9. Cui, H. et al. 2020. Monocyte-derived alveolar macrophage Apolipoprotein E participates in pulmonary fibrosis resolution. JCI Insight. (https://insight.jci.org/articles/view/134539)

  10. Kimura, Y. et al. 2020. Soluble Uric Acid Promotes Atherosclerosis via AMPK (AMP-Activated Protein Kinase)-Mediated Inflammation. Arteriosclerosis, Thrombosis, and Vascular Biology. 40:570–582. (https://www.ahajournals.org/doi/full/10.1161/ATVBAHA.119.313224)

  11. Blanco, S. et al. 2020. Hyaluronate Nanoparticles as a Delivery System to Carry Neuroglobin to the Brain after Stroke. Pharmaceutics  (https://doi.org/10.3390/pharmaceutics12010040)

  12. Fallet, B. et al. 2020. Chronic Viral Infection Promotes Efficient Germinal Center B Cell Responses. Cell Reports. 30, 1013–1026. (https://www.sciencedirect.com/science/article/pii/S2211124719316730#!)

  13. Kerby, A. et al. 2020. Placental Morphology and Cellular Characteristics in Stillbirths in Women With Diabetes and Unexplained Stillbirths. Archives of Pathology & Laboratory Medicine. (https://www.archivesofpathology.org/doi/10.5858/arpa.2019-0524-OA)

  14. Imamura, T. et al. 2020. Insulin deficiency promotes formation of toxic amyloid-β42 conformer co-aggregating with hyper-phosphorylated tau oligomer in an Alzheimer's disease model. Neurobiology of Disease 137:1047392. (https://www.sciencedirect.com/science/article/pii/S0969996120300140)

  15. Bärnthaler, T. et al. 2020. Inhibiting eicosanoid degradation exertsantifibrotic effects in a pulmonary fibrosismouse model and human tissue. Journal of Allergy and Clinical Immunology. Volume 145, Issue 3,, Pages 818-833.e11 (https://www.sciencedirect.com/science/article/pii/S0091674919316252#!)

  16. Vinton, C.L. et al. 2019. Simian Immunodeficiency Virus Infection of Rhesus Macaques Results in Delayed Zika Virus Clearance. mBio (https://doi.org/10.1128/mBio.02790-19)

  17. Oswald, D.M., Jones, M.B., Cobb, B.A. 2019.  Modulation of hepatocyte sialylation drives spontaneous fatty liver disease and inflammation. Glycobiology (https://academic.oup.com/glycob/advance-article-abstract/doi/10.1093/glycob/cwz096/5628931)

  18. Ushioda, W. et al. 2019. Neuropathology in Neonatal Mice After Experimental Coxsackievirus B2 Infection Using a Prototype Strain, Ohio-1. Journal of Neuropathology & Experimental Neurology  (https://academic.oup.com/jnen/advance-article-abstract/doi/10.1093/jnen/nlz124/5679985)

  19. Ibrahim, M. et al. 2019. Once Daily Pregabalin Eye Drops for Management of Glaucoma. ACS Nano 2019, 13, 13728−13744. (https://www.sciencedirect.com/science/article/pii/S0969996120300140)

  20. Bencze, J., et al. 2019. Neuropathological characterization of Lemur tyrosine kinase 2 (LMTK2) in Alzheimer’s disease and neocortical Lewy body disease. Scientific Reports (https://www.nature.com/articles/s41598-019-53638-9)

  21. Davies, S.P. et al. 2019. Hepatocytes Delete Regulatory T Cells by Enclysis, a CD4+ T Cell Engulfment Process. Cell Reports (https://www.sciencedirect.com/science/article/pii/S2211124719312653)

  22. Liebers, J. et al. 2019. 3D image analysis reveals differences of CD30 positive cells and network formation in reactive and malignant human lymphoid tissue (classical Hodgkin Lymphoma). PLoS One (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6812863/)

  23. Lecocq, Q. et al. 2019. Noninvasive Imaging of the Immune Checkpoint LAG-3 Using Nanobodies, from Development to Pre-Clinical Use. Biomolecules (https://www.mdpi.com/2218-273X/9/10/548/htm)

  24. Motoike, S. et al. 2019. Clumps of Mesenchymal Stem Cell/Extracellular Matrix Complexes Generated with Xeno-Free Conditions Facilitate Bone Regeneration via Direct and Indirect Osteogenesis. International Journal of Molecular Sciences (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6720767/)

  25. Wilson, M.R. et al. 2019. ARID1A and PI3-kinase pathway mutations in the endometrium drive epithelial transdifferentiation and collective invasion. Nature Communications (https://www.nature.com/articles/s41467-019-11403-6)

  26. Nagai-Okatani, C. et al. 2019. Wisteria floribunda agglutinin staining for the quantitative assessment of cardiac fibrogenic activity in a mouse model of dilated cardiomyopathy. Laboratory Investigation (https://www.nature.com/articles/s41374-019-0279-9)

  27. Sheller-Miller, S. et al. 2019. Cyclic-recombinase-reporter mouse model to determine exosome communication and function during pregnancy. American Journal of Obstetrics and Gynecology (https://www.sciencedirect.com/science/article/abs/pii/S0002937819307744#!)

  28. Abe, H. et al. 2019. Correlation between platelet thrombus formation on collagen-coated beads and platelet aggregation induced by ADP. Transfusion and Apheresis Science (https://www.trasci.com/article/S1473-0502(19)30090-4/abstract)

  29. Singh, B. et al. 2019. Tau is required for progressive synaptic and memory deficits in a transgenic mouse model of α-synucleinopathy. Acta Neuropathologica (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6778173/)

  30. Chafe, S.C. et al. 2019. Targeting hypoxia-induced carbonic anhydrase IX enhances immune-checkpoint blockade locally and systemically. Cancer Immunol Res (http://cancerimmunolres.aacrjournals.org/content/early/2019/05/31/2326-6066.CIR-18-0657)

  31. Rhodes, S. et al. 2019. Cdkn2a (Arf) loss drives NF1-associated atypical neurofibroma and malignant transformation. Human Molecular Genetics (https://academic.oup.com/hmg/article/28/16/2752/5489753)

  32. Su, X. et al. 2019. Imaging and Tracking Stem Cell Engraftment in Ischemic Hearts by Near-Infrared Fluorescent Protein (iRFP) Labeling. Methods in Molecular Biology (https://link.springer.com/protocol/10.1007%2F7651_2019_226)

  33. Rodgers, H.M. et al. 2019. Dopamine D1 and D3 receptor modulators restore morphine analgesia and prevent opioid preference in a model of neuropathic pain. Neuroscience (https://www.sciencedirect.com/science/article/abs/pii/S0306452219301927)

  34. Yoon, J.H., Li, M., Basile, J.R., Lin, Y 2018. Computer-assisted analysis of immunohistological parameters in oral giant cell granulomas, Oral Diseases (https://doi.org/10.1111/odi.13022)

  35. Nishimura, A. et al. 2018. Hypoxia-induced interaction of filamin with Drp1 causes mitochondrial hyperfission–associated myocardial senescence. Science Signaling (https://stke.sciencemag.org/content/11/556/eaat5185.long)

  36. Su Y, Hou Y, Wang Q. 2018. The enhanced replication of an S-intact PEDV during coinfection with an S1 NTD-del PEDV in piglets, Veterinary Microbiology (https://doi.org/10.1016/j.vetmic.2018.11.025)

  37. Soontornniyomkij, V. et al. 2018. Association of antiretroviral therapy with brain aging changes among HIV-infected adults. AIDS (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6115290/)

  38. Du, H. et al. 2018. A novel mouse model of hemangiopericytoma due to loss of Tsc2. Human Mol. Gen. (https://doi.org/10.1093/hmg/ddy289)

  39. Boucher, J.M. et al. 2018. Rab27a regulates human perivascular adipose progenitor cell differentiation. Cardiovasc Drugs Ther. (https://doi.org/10.1007/s10557-018-6813-y)

Comparison with other autofluorescence reducing agents

Whereas most methods for reducing tissue autofluorescence act primarily on lipofuscin granules, the Vector® TrueVIEW™ quencher targets fluorescence from non-lipofuscin sources, including aldehyde fixation, red blood cells, and structural elements, such as collagen and elastin. It provides a clear, unambiguous “true view” of target antigen localization, even in problematic tissues, such as kidney, spleen and pancreas.

Comparisons with other commercials and “home brew” approaches show that Vector® TrueVIEW™ is easier to use and more effective. The images below show the results of side-by-side comparisons on serial sections of formalin-fixed, paraffin embedded human pancreas visualized using a standard fluorescein (green) filter. No specific immunofluorescence staining was conducted.

No Treatment (Endogenous autofluorescence)

TrueVIEW Quencher Treated

Competitor and other autofluorescence treatments

Company A

Company B

Company C

Copper Sulfate Solution

Sodium Borohydride


Sudan Black B

 

Listen to the podcast
LISTEN to the podcast by Timothy Karpishin, PhD, Director of Chemistry at Vector Laboratories, Inc. describing TrueVIEW™ Autofluorescence Quenching Kit at the 43rd Annual NSH Symposium/Convention.

Download the poster
DOWNLOAD the poster describing TrueVIEW™ Autofluorescence Quenching Kit presented at the 43rd Annual NSH Symposium/Convention.

Download the brochure for more information
Learn more about the Vector® TrueVIEW™ Autofluorescence Quenching Kit by downloading the brochure.

Easy to Use

Following completion of immunofluorescence staining:

TrueView Steps

Mode of Action