Vector® TrueVIEW® Autofluorescence Quenching Kit

Dramatically reduce autofluorescence to reveal true immunofluorescence

In tissue sections, autofluorescence is the unwanted fluorescence that can make it difficult or impossible to distinguish antigen-specific signal from non-specific background noise. The novel, patent-pending TrueVIEW Autofluorescence Quenching Kit specifically binds and quenches autofluorescent elements from non-lipofuscin sources, significantly enhancing signal-to-noise in most immunofluorescence assays—even in the most challenging tissues.

WITHOUT Treatment

FFPE sections of human prostate not treated with TrueVIEW™ Autofluorescence Quenching Kit show significant fluorescence

WITH TrueVIEW Quencher

FFPE sections of human prostate treated with TrueVIEW™ Autofluorescence Quenching Kit show a complete absence of fluorescence

 

Reduction of autofluorescence in human spleen using the TrueVIEW Autofluorescence Quenching Kit. Human spleen sections (FFPE) stained using mouse anti-CD20 (red) and rabbit anti-Ki67 (green) primary antibodies detected with VectaFluor™ Duet kit (DK-8818). Note significant reduction of autofluorescence in the treated section.

Our customers have great things to say about the TrueVIEW Autofluorescence Kit!

 
 

Why TrueVIEW Quencher?

  • Specific reduction of autofluorescence from non-lipofuscin sources
  • Easy-to-use, one-step method
  • Quick 5 min incubation
  • Compatible with a wide selection of fluorophores
  • Compatible with standard epifluorescence and confocal laser microscopes
 

Comparison with other autofluorescence reducing agents

Whereas most methods for reducing tissue autofluorescence act primarily on lipofuscin granules, the TrueVIEW quencher targets fluorescence from non-lipofuscin sources, including aldehyde fixation, red blood cells, and structural elements, such as collagen and elastin. It provides a clear, unambiguous “true view” of target antigen localization, even in problematic tissues, such as kidney, spleen, and pancreas.

Comparisons with other commercial and “home brew” approaches show that TrueVIEW is easier to use and more effective at reducing autofluorescence. The images below show the results of side-by-side comparisons on serial sections of formalin-fixed, paraffin embedded human pancreas visualized using a standard fluorescein (green) filter. No specific immunofluorescence staining was conducted.

No Treatment (Endogenous autofluorescence)

formalin-fixed, paraffin embedded (FFPE) human pancreas visualized using a standard fluorescein (green) filter without treatment by Trueview autofluorescence reducing agent

TrueVIEW Quencher Treated

formalin-fixed, paraffin embedded (FFPE) human pancreas visualized using a standard fluorescein (green) filter showing a complete absence of autofluorescence after treatment with Trueview autofluorescence reducing agent

Competitive treatments for dealing with autofluorescence

Company A

Side by side comparison of competitor autofluorescence treatments as compared with Trueview autofluorescence reducing agent

Company B

Side by side comparison of competitor autofluorescence treatments as compared with Trueview autofluorescence reducing agent

Company C

Side by side comparison of competitor autofluorescence treatments as compared with Trueview autofluorescence reducing agent
 

Alternative treatments for dealing with autofluorescence

Copper Sulfate Solution

Side by side comparison of alternative autofluorescence treatment products as compared with Trueview autofluorescence reducing agent

Sodium Borohydride

Side by side comparison of alternative autofluorescence treatment products as compared with Trueview autofluorescence reducing agent

Sudan Black B

Side by side comparison of alternative autofluorescence treatment products as compared with Trueview autofluorescence reducing agent

 

Additional resources available from Vector Labs

National Society for Histotechnology (NSH) Podcast highlighting a simple method for effective reduction in tissue autofluorescence

Listen to the podcast
LISTEN to the podcast by Timothy Karpishin, PhD, Director of Chemistry at Vector Laboratories, Inc. describing TrueVIEW Autofluorescence Quenching Kit at the 43rd Annual NSH Symposium/Convention.

Poster describing TrueVIEW™ Autofluorescence Quenching Kit as presented at the 43rd Annual NSH Symposium/Convention

Download the poster
DOWNLOAD the poster describing TrueVIEW Autofluorescence Quenching Kit presented at the 43rd Annual NSH Symposium/Convention.

Brochure for the TrueVIEW™ Autofluorescence Quenching Kit available from Vector Labs

Download the brochure for more information
Learn more about the TrueVIEW Autofluorescence Quenching Kit by downloading the brochure.

The TrueVIEW Autofluorescence Kit is easy to use

Following completion of immunofluorescence staining:

Step by step explanation of the TrueVIEW™ Autofluorescence Kit showing how easy it is to use

Mode of Action

 Diagram of the TrueVIEW™ Autofluorescence Kit showing its mode of action

Product Details & Ordering Information

Product Catalog Number Applications Unit Size

TrueVIEW Autofluorescence Quenching Kit

SP-8400-15 Immunofluorescence, In situ hybridization 15 mL
TrueVIEW Autofluorescence Quenching Kit with DAPI SP-8500-15 Immunofluorescence, In situ hybridization 15 mL
VECTASHIELD® Vibrance™ Antifade Mounting Medium H-1700 Immunofluorescence, In situ hybridization, Cellular Imaging 2 mL, 10 mL
VECTASHIELD Vibrance with DAPI Antifade Mounting Medium H-1800 Immunofluorescence, In situ hybridization, Cellular Imaging 2 mL, 10 mL

 

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