Vector® TrueVIEW™ Autofluorescence Quenching Kit
Dramatically reduce autofluorescence to reveal true immunofluorescence
In tissue sections, autofluorescence is the unwanted fluorescence that can make it difficult or impossible to distinguish antigen-specific signal from non-specific background noise. The novel, patent-pending Vector® TrueVIEW™ Autofluorescence Quenching Kit specifically binds and quenches autofluorescent elements from non-lipofuscin sources, significantly enhancing signal-to-noise in most immunofluorescence assays—even in the most challenging tissues.
WITH Vector® TrueVIEW™ Quencher
Our customers have great things to say about the Vector® TrueVIEW™ Autofluorescence Kit!
Why TrueVIEW™ Quencher?
- Specific reduction of autofluorescence from non-lipofuscin sources
- Easy-to-use, one-step method
- Quick 5 min incubation
- Compatible with a wide selection of fluorophores
- Compatible with standard epifluorescence and confocal laser microscopes
Comparison with other autofluorescence reducing agents
Whereas most methods for reducing tissue autofluorescence act primarily on lipofuscin granules, the Vector® TrueVIEW™ quencher targets fluorescence from non-lipofuscin sources, including aldehyde fixation, red blood cells, and structural elements, such as collagen and elastin. It provides a clear, unambiguous “true view” of target antigen localization, even in problematic tissues, such as kidney, spleen, and pancreas.
Comparisons with other commercial and “home brew” approaches show that Vector® TrueVIEW™ is easier to use and more effective at reducing autofluorescence. The images below show the results of side-by-side comparisons on serial sections of formalin-fixed, paraffin embedded human pancreas visualized using a standard fluorescein (green) filter. No specific immunofluorescence staining was conducted.
No Treatment (Endogenous autofluorescence)
TrueVIEW™ Quencher Treated
Competitive treatments for dealing with autofluorescence
Alternative treatments for dealing with autofluorescence
Copper Sulfate Solution
Sudan Black B
Additional resources available from Vector Labs
Listen to the podcast
LISTEN to the podcast by Timothy Karpishin, PhD, Director of Chemistry at Vector Laboratories, Inc. describing TrueVIEW™ Autofluorescence Quenching Kit at the 43rd Annual NSH Symposium/Convention.
Download the poster
DOWNLOAD the poster describing TrueVIEW™ Autofluorescence Quenching Kit presented at the 43rd Annual NSH Symposium/Convention.
Download the brochure for more information
Learn more about the Vector® TrueVIEW™ Autofluorescence Quenching Kit by downloading the brochure.
The TrueVIEW™ Autofluorescence Kit is easy to use
Following completion of immunofluorescence staining:
Mode of Action
Product Details & Ordering Information
|Product||Catalog Number||Applications||Unit Size|
TrueVIEW® Autofluorescence Quenching Kit
|SP-8400||Immunofluorescence, In situ hybridization||15 mL|
|TrueVIEW® Autofluorescence Quenching Kit with DAPI||SP-8500||Immunofluorescence, In situ hybridization||15 mL|
|VECTASHIELD® Vibrance™ Antifade Mounting Medium||H-1700||Immunofluorescence, In situ hybridization, Cellular Imaging||2 mL, 10 mL|
|VECTASHIELD® Vibrance™ with DAPI Antifade Mounting Medium||H-1800||Immunofluorescence, In situ hybridization, Cellular Imaging||2 mL, 10 mL|
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