Dramatically Reduce Autofluorescence

Vector® TrueVIEW™ Autofluorescence Quenching Kit

Reveal true immunofluorescence—even in the most challenging tissues

In tissue sections, autofluorescence is the unwanted fluorescence that can make it difficult or impossible to distinguish antigen-specific signal from non-specific background. The novel, patent-pending Vector® TrueVIEW™ Autofluorescence Quenching Kit specifically binds and quenches autofluorescent elements from non-lipofuscin sources, significantly enhancing signal-to-noise in most immunofluorescence assays.

Why TrueVIEW™ Quencher?
  • Specific reduction of autofluorescence from non-lipofuscin sources
  • Easy-to-use, one-step method
  • Quick 5 min incubation
  • Compatible with a wide selection of fluorophores
  • Compatible with standard epifluorescence and confocal laser microscopes

WITHOUT Treatment

Human Spleen not Treated with TrueVIEW™ Autofluorescence Quenching Kit

WITH Vector® TrueVIEW™ Quencher

Reduction of Autofluorescence in Human Spleen using the TrueVIEW™ Autofluorescence Quenching Kit


Reduction of autofluorescence in human spleen using the TrueVIEW™ Autofluorescence Quenching Kit. Human spleen sections (FFPE) stained using mouse anti-CD20 (red) and rabbit anti-Ki67 (green) primary antibodies detected with VectaFluor™ Duet kit (DK-8818). Note significant reduction of autofluorescence in the treated section.

Our customers have great things to say about the Vector® TrueVIEW Autofluorescence Kit!


  1. de Barros Mucci, D, et al. 2020. Impact of maternal obesity on placental transcriptome and morphology associated with fetal growth restriction in mice. International Journal of Obesity. (https://www.nature.com/articles/s41366-020-0561-3)

  2. Cui, H. et al. 2020. Monocyte-derived alveolar macrophage Apolipoprotein E participates in pulmonary fibrosis resolution. JCI Insight. (https://insight.jci.org/articles/view/134539)

  3. Blanco, S. et al. 2020. Hyaluronate Nanoparticles as a Delivery System to Carry Neuroglobin to the Brain after Stroke. Pharmaceutics  (https://doi.org/10.3390/pharmaceutics12010040)

  4. Fallet, B. et al. 2020. Chronic Viral Infection Promotes Efficient Germinal Center B Cell Responses. Cell Reports. 30, 1013–1026. (https://www.sciencedirect.com/science/article/pii/S2211124719316730#!)

  5. Kerby, A. et al. 2020. Placental Morphology and Cellular Characteristics in Stillbirths in Women With Diabetes and Unexplained Stillbirths. Archives of Pathology & Laboratory Medicine. (https://www.archivesofpathology.org/doi/10.5858/arpa.2019-0524-OA)

  6. Imamura, T. et al. 2020. Insulin deficiency promotes formation of toxic amyloid-β42 conformer co-aggregating with hyper-phosphorylated tau oligomer in an Alzheimer's disease model. Neurobiology of Disease 137:1047392. (https://www.sciencedirect.com/science/article/pii/S0969996120300140)

  7. Vinton, C.L. et al. 2019. Simian Immunodeficiency Virus Infection of Rhesus Macaques Results in Delayed Zika Virus Clearance. mBio (https://doi.org/10.1128/mBio.02790-19)

  8. Oswald, D.M., Jones, M.B., Cobb, B.A. 2019.  Modulation of hepatocyte sialylation drives spontaneous fatty liver disease and inflammation. Glycobiology (https://academic.oup.com/glycob/advance-article-abstract/doi/10.1093/glycob/cwz096/5628931)

  9. Ushioda, W. et al. 2019. Neuropathology in Neonatal Mice After Experimental Coxsackievirus B2 Infection Using a Prototype Strain, Ohio-1. Journal of Neuropathology & Experimental Neurology  (https://academic.oup.com/jnen/advance-article-abstract/doi/10.1093/jnen/nlz124/5679985)

  10. Ibrahim, M. et al. 2019. Once Daily Pregabalin Eye Drops for Management of Glaucoma. ACS Nano 2019, 13, 13728−13744. (https://www.sciencedirect.com/science/article/pii/S0969996120300140)

  11. Bencze, J., et al. 2019. Neuropathological characterization of Lemur tyrosine kinase 2 (LMTK2) in Alzheimer’s disease and neocortical Lewy body disease. Scientific Reports (https://www.nature.com/articles/s41598-019-53638-9)

  12. Davies, S.P. et al. 2019. Hepatocytes Delete Regulatory T Cells by Enclysis, a CD4+ T Cell Engulfment Process. Cell Reports (https://www.sciencedirect.com/science/article/pii/S2211124719312653)

  13. Liebers, J. et al. 2019. 3D image analysis reveals differences of CD30 positive cells and network formation in reactive and malignant human lymphoid tissue (classical Hodgkin Lymphoma). PLoS One (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6812863/)

  14. Lecocq, Q. et al. 2019. Noninvasive Imaging of the Immune Checkpoint LAG-3 Using Nanobodies, from Development to Pre-Clinical Use. Biomolecules (https://www.mdpi.com/2218-273X/9/10/548/htm)

  15. Motoike, S. et al. 2019. Clumps of Mesenchymal Stem Cell/Extracellular Matrix Complexes Generated with Xeno-Free Conditions Facilitate Bone Regeneration via Direct and Indirect Osteogenesis. International Journal of Molecular Sciences (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6720767/)

  16. Wilson, M.R. et al. 2019. ARID1A and PI3-kinase pathway mutations in the endometrium drive epithelial transdifferentiation and collective invasion. Nature Communications (https://www.nature.com/articles/s41467-019-11403-6)

  17. Nagai-Okatani, C. et al. 2019. Wisteria floribunda agglutinin staining for the quantitative assessment of cardiac fibrogenic activity in a mouse model of dilated cardiomyopathy. Laboratory Investigation (https://www.nature.com/articles/s41374-019-0279-9)

  18. Sheller-Miller, S. et al. 2019. Cyclic-recombinase-reporter mouse model to determine exosome communication and function during pregnancy. American Journal of Obstetrics and Gynecology (https://www.sciencedirect.com/science/article/abs/pii/S0002937819307744#!)

  19. Abe, H. et al. 2019. Correlation between platelet thrombus formation on collagen-coated beads and platelet aggregation induced by ADP. Transfusion and Apheresis Science (https://www.trasci.com/article/S1473-0502(19)30090-4/abstract)

  20. Singh, B. et al. 2019. Tau is required for progressive synaptic and memory deficits in a transgenic mouse model of α-synucleinopathy. Acta Neuropathologica (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6778173/)

  21. Chafe, S.C. et al. 2019. Targeting hypoxia-induced carbonic anhydrase IX enhances immune-checkpoint blockade locally and systemically. Cancer Immunol Res (http://cancerimmunolres.aacrjournals.org/content/early/2019/05/31/2326-6066.CIR-18-0657)

  22. Rhodes, S. et al. 2019. Cdkn2a (Arf) loss drives NF1-associated atypical neurofibroma and malignant transformation. Human Molecular Genetics (https://academic.oup.com/hmg/article/28/16/2752/5489753)

  23. Rodgers, H.M. et al. 2019. Dopamine D1 and D3 receptor modulators restore morphine analgesia and prevent opioid preference in a model of neuropathic pain. Neuroscience (https://www.sciencedirect.com/science/article/abs/pii/S0306452219301927)

  24. Yoon, J.H., Li, M., Basile, J.R., Lin, Y 2018. Computer-assisted analysis of immunohistological parameters in oral giant cell granulomas, Oral Diseases (https://doi.org/10.1111/odi.13022)

  25. Nishimura, A. et al. 2018. Hypoxia-induced interaction of filamin with Drp1 causes mitochondrial hyperfission–associated myocardial senescence. Science Signaling (https://stke.sciencemag.org/content/11/556/eaat5185.long)

  26. Su Y, Hou Y, Wang Q. 2018. The enhanced replication of an S-intact PEDV during coinfection with an S1 NTD-del PEDV in piglets, Veterinary Microbiology (https://doi.org/10.1016/j.vetmic.2018.11.025)

  27. Soontornniyomkij, V. et al. 2018. Association of antiretroviral therapy with brain aging changes among HIV-infected adults. AIDS (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6115290/)

  28. Du, H. et al. 2018. A novel mouse model of hemangiopericytoma due to loss of Tsc2. Human Mol. Gen. (https://doi.org/10.1093/hmg/ddy289)

  29. Boucher, J.M. et al. 2018. Rab27a regulates human perivascular adipose progenitor cell differentiation. Cardiovasc Drugs Ther. (https://doi.org/10.1007/s10557-018-6813-y)

Comparison with other autofluorescence reducing agents

Whereas most methods for reducing tissue autofluorescence act primarily on lipofuscin granules, the Vector® TrueVIEW™ quencher targets fluorescence from non-lipofuscin sources, including aldehyde fixation, red blood cells, and structural elements, such as collagen and elastin. It provides a clear, unambiguous “true view” of target antigen localization, even in problematic tissues, such as kidney, spleen and pancreas.

Comparisons with other commercials and “home brew” approaches show that Vector® TrueVIEW™ is easier to use and more effective. The images below show the results of side-by-side comparisons on serial sections of formalin-fixed, paraffin embedded human pancreas visualized using a standard fluorescein (green) filter. No specific immunofluorescence staining was conducted.

No Treatment (Endogenous autofluorescence)

TrueVIEW Quencher Treated

Competitor and other autofluorescence treatments

Company A

Company B

Company C

Copper Sulfate Solution

Sodium Borohydride

Sudan Black B


Listen to the podcast
LISTEN to the podcast by Timothy Karpishin, PhD, Director of Chemistry at Vector Laboratories, Inc. describing TrueVIEW™ Autofluorescence Quenching Kit at the 43rd Annual NSH Symposium/Convention.

Download the poster
DOWNLOAD the poster describing TrueVIEW™ Autofluorescence Quenching Kit presented at the 43rd Annual NSH Symposium/Convention.

Download the brochure for more information
Learn more about the Vector® TrueVIEW™ Autofluorescence Quenching Kit by downloading the brochure.

Easy to Use

Following completion of immunofluorescence staining:

TrueView Steps

Mode of Action