Vector Labs is closed to observe Juneteenth (US Holiday) on Wednesday, June 19th.
We will be back in the office on Thursday. We will respond to emails upon our return. Have a wonderful day.

O-propargyl-puromycine (OPP) (CCT-1407)

[text-backorder]

Description

O-propargyl-puromycin (OPP) is an alkyne analog of puromycin that is efficiently incorporated into newly translated proteins, which are rapidly turned over by the proteasome and can be visualized or captured by copper(I)-catalyzed azide-alkyne cycloaddition. Unlike methionine analogs, OPP does not require methionine-free conditions and, uniquely, can be used to label and assay nascent proteins in whole organisms.

Specifications

Unit Size5 mg, 25 mg, 100 mg
Molecular weight495.54
Chemical compositionC24H29N7O5
CAS1416561-90-4
SolubilityDMSO, DMF
Purity>95% (HPLC)
AppearanceOff-white to slightly grey crystalline
Storage Conditions-20°C.
Shipping ConditionsAmbient temperature

Selected References

  1. Leach, R. W., et al. (2021). Activity-based RNA-modifying enzyme probing reveals DUS3L-mediated dihydrouridylation. Nat Chem Biol.17 (11), 1178-1187. [PubMed]
  2. Morral, C., et al. (2020). Zonation of Ribosomal DNA Transcription Defines a Stem Cell Hierarchy in Colorectal Cancer. Cell Stem Cell.26 (6), 845-861.e12. [PubMed]
  3. Uchiyama, J., et al. (2020). Quantitative nascent proteome profiling by dual pulse labeling with O-propargyl-puromycin and stable isotope labeled amino acids. The Journal of Biochemistry10, 1093. [Oxford Academic]
  4. Lui, J., et al. (2012). Imaging protein synthesis in cells and tissues with an alkyne analog of puromycin. PNAS109, 413-8. [PubMed]
  5. Forester, C.M., et al. (2018). Revealing nascent proteomics in signaling pathways and cell differentiation.PNAS115, 2353-8. [PubMed]
  6. Tong, M., et al. (2020). Effective Method for Accurate and Sensitive Quantitation of Rapid Changes of Newly Synthesized Proteins. Anal. Chem.92(14), 10048-57. [PubMed]

Applicable patents and legal notices are available at legal notices.

How do I Request a Quote?

To request a quote for products:

  1.  Log in to VectorLabs.com
  2.  Add the items you wish to quote to the cart;
  3.  View your cart;
  4.  Click on the “Request a Quote” button found below the items in your cart;
  5.  Download your quote