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L-Azidohomoalanine (AHA) (CCT-1066)

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Description

L-Azidohomoalanine (AHA) is an amino acid analog of methionine that contains a very small modification, specifically an azido moiety that can be fed to cultured cells and incorporated into proteins during active protein synthesis. Detection utilizes the chemoselective ligation or “click ” reaction between an azide and an alkyne or cyclooctyne. The azido-modified protein is detected with either fluorescent alkyne or biotin alkyne. Detection sensitivity with these reagents in 1-D gels and Western blots is in the low femtomole range and compatible with downstream LC-MS⁄MS and MALDI MS analysis.

Specifications

Unit Size25 mg, 100 mg, 1000 mg, 5 g
Molecular weight180.59 (HCl salt)
Chemical compositionC4H9ClN4O2 (HCl salt)
CAS942518-29-8
SolubilityWater, DMSO, DMF
Purity>95% (H NMR)
AppearanceWhite crystalline
Storage Conditions-20°C.
Shipping ConditionsAmbient temperature

Selected References

  1. Loebel , C., et al. (2022). Metabolic labeling of secreted matrix to investigate cell-material interactions in tissue engineering and mechanobiology. Nat Protoc.10.1038, Online ahead of print. [PubMed]
  2. Bruna, R. E., et al. (2021). Limitation of phosphate assimilation maintains cytoplasmic magnesium homeostasis. Proc Natl Acad Sci U S A.118 (11), e2021370118. [PubMed]
  3. Costello, A., et al. (2021). Directed evolution of rRNA improves translation kinetics and recombinant protein yield. Nat Commun.12 (1), 5638. [PubMed]
  4. Kwong, J. Q., et al. (2021). Mitochondrial functional resilience after TFAM ablation in the adult heart. Am J Physiol Cell Physiol.320 (6), C929-C942. [PubMed]
  5. Heybrock, S., et al. (2021). S-palmitoylation determines TMEM55B-dependent positioning of lysosomes. J Cell Sci.135 (5), jcs258566. [PubMed]
  6. Coates, H. W. et al. (2021). The mammalian cholesterol synthesis enzyme squalene monooxygenase is proteasomally truncated to a constitutively active form. J Biol Chem., Online ahead of print. [PubMed]
  7. Morral, C., et al. (2020). Zonation of Ribosomal DNA Transcription Defines a Stem Cell Hierarchy in Colorectal Cancer. Cell Stem Cell.26 (6), 845-861.e12. [PubMed]
  8. Smeekens, J. M., et al. (2020). Systematic quantification of the dynamics of newly synthesized proteins unveiling their degradation pathways in human cells. Chem Sci.11 (13), 3557-3568. [PubMed]
  9. Tao, W.A., et al. (2019). Identification and Quantification of Newly Synthesized Proteins Using Mass Spectrometry‐Based Chemical Proteomics. Mass Spectrometry‐Based Chemical Proteomics. [PubMed]
  10. Dumont, A. A., et al. (2018). ADAP1 limits neonatal cardiomyocyte hypertrophy by reducing integrin cell surface expression. Sci Rep.8 (1), 13605. [PubMed]

Applicable patents and legal notices are available at legal notices.

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