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IR 650 DBCO (FP-1506)

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Description

IR 650 DBCO (IRDye® 650 DBCO analog) is a water-soluble, bright, far-red-fluorescent probe with excitation ideally suited for the 633 nm or 647 nm laser lines. A significant advantage to using long wavelength dyes such as Cy5® Dye or Alexa Fluor® 647 dye over other fluorophores is the low autofluorescence of biological specimens in this region of the spectrum.

IR 650 DBCO is a bright, far-red-fluorescent, pH insensitive azide-reactive probe routinely used for imaging of azide-containing biomolecules without the need for copper catalyst. IR 650 DBCO reacts with azides via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. In application where the presence of copper is a concern IR 650 DBCO is an ideal alternative to copper requiring fluorescent alkynes.

Specifications

Unit Size1 mg, 5 mg, 25 mg, 100 mg
ReactivityAzide, picolyl azide
Abs/Em Maxima651/668 nm
Extinction coefficient230,000 cm-1M-1
SolubilityWater, DMSO, DMF
Spectrally similar dyesCy5, Alexa Fluor® 647, DyLight® 649,
Molecular weight1193.43
Storage Conditions-20°C.
Shipping ConditionsAmbient temperature

Abs/Em Spectra

Cy5

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