Desthiobiotin is a single-ring, sulfur-free analog of biotin that binds to streptavidin with nearly equal specificity but less affinity than biotin (Kd = 1011 vs. 1015M, respectively). Consequently, desthiobiotinylated proteins can be eluted readily and specifically from streptavidin affinity resin using mild conditions based on competitive displacement with free biotin. For pull-down experiments with biological samples, this soft-release characteristic of desthiobiotin also helps to minimize co-purification of endogenous biotinylated molecules, which remain bound to streptavidin upon elution of the target protein complexes with free biotin.
| Unit Size | 5 mg, 25 mg, 100 mg |
|---|---|
| Molecular weight | 427.23 |
| Chemical composition | C21H37N3O6 |
| CAS | 1951424-89-7 |
| Solubility | DMSO, DMF |
| Purity | >95% (HPLC) |
| Appearance | Colorless to slightly yellow oil |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient temperature |
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