Cy5 Picolyl Azide is an advanced fluorescent probe that incorporates a copper-chelating motif to raise the effective concentration of Cu(I) at the reaction site to boost the efficiency of the CuAAC reaction, resulting in a faster and more biocompatible CuAAC labeling. Up to 40-fold increase of signal intensity, compared to conventional azides, was reported (see Selected References).
In addition, the use picolyl azides instead of conventional azides allows for at least a tenfold reduction in the concentration of the copper catalyst without sacrificing the efficiency of labeling, significantly improving biocompatibility of CuAAC labeling protocol.
In summary, the introduction of a copper-chelating motif into azide probe leads to a substantial increase in the sensitivity and reduced cell toxicity of CuAAC detection alkyne-tagged biomolecules. This will be of special value for the detection of low abundance targets or living system imaging.
Cy5 Picolyl Azide is a water-soluble, pH-insensitive from pH 4 to pH 10, far-red-fluorescent probe with excitation ideally suited for the 633 nm or 647 nm laser lines. Cy5 dye is structurally similar, and spectrally is almost identical to Alexa Fluor® 647, CF® 647 Dye, or any other Cyanine5 based fluorescent dyes.
Unit Size | 1 mg, 5 mg, 25 mg |
---|---|
Abs/Em Maxima | 647/663 nm |
Extinction Coefficient | 250,000 |
Flow Cytometry Laser Line | 633 nm or 647 nm |
Microscopy Laser Line | 633 nm or 647 nm |
Spectrally Similar Dyes | Alexa Fluor® 647, CF® 647, DyLight® 649 |
Molecular weight | 953.15 (protonated) |
CAS | N/A |
Solubility | Water, DMSO, DMF |
Purity | >95% (HPLC) |
Appearance | Blue solid |
Storage Conditions | -20°C. Desiccate |
Shipping Conditions | Ambient temperature |
1. Jiang, H., et al. (2014). Monitoring Dynamic Glycosylation in Vivo Using Supersensitive Click Chemistry. Bioconjugate Chem.,, 25, 698-706. [PubMed]
2. Uttamapinant, C., et al. (2012). Fast, Cell-Compatible Click Chemistry with Copper-Chelating Azides for Biomolecular Labeling. Angew. Chem. Int. Ed,., 51, 5852-56. [PubMed]
3. Gaebler, A.,et al. (2016). A highly sensitive protocol for microscopy of alkyne lipids and fluorescently tagged or immunostained proteins. J. Lipid. Res., 57, 1934-47. [PubMed]
4. Knutson, S.D.,et al. (2020). Chemical Profiling of A‐to‐I RNA Editing Using a Click‐Compatible Phenylacrylamide Chem. Eur.J., 26, 9874-78. [PubMed]
Applicable patents and legal notices are available at legal notices.
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