Vector Laboratories is closed for the President’s Day on Monday, February 19th. We will be back in the office on Tuesday, February 20th.
We will respond to emails upon our return. Have a wonderful day.

Click-&-Go® Protein Reaction Buffer Kit (CCT-1262)

[text-backorder]
Click-&-Go® Protein Reaction Buffer Kit provides researchers everything required to perform the click reaction on azide or alkyne tagged proteins with the corresponding click detection reagent for subsequent downstream analysis. Each kit includes sufficient material to perform 25 reactions for subsequent analysis by gel electrophoresis, western blot or mass spectrometry.

Description

Click-&-Go Click Chemistry Reaction Buffer Kit provides researchers who have biomolecules labeled with an azide or alkyne and the corresponding click detection reagent with all the necessary reagents to perform an up to 25 copper-catalyzed click reactions for subsequent analysis by gel electrophoresis, western blot or mass spectrometry.

1262 scheme 01

The kit utilizes a copper chelating reagent to stabilize a catalytically active Cu(I) to accelerate the rate of CuAAC. The use of copper-chelating ligand also minimizes amount of free copper in solution, thus limiting the generation of reactive oxygen species (ROS) and protecting against undesired oxidative damage side reactions to proteins (e.g., GFP, RPE), nucleic acids (e.g., RNA, oligos), and other biomolecules.

Specifications

Unit Size1 kit
Number of Reactions
25
Storage Conditions4C
Shipping ConditionsAmbient temperature

Selected References

  1. Bruna, R. E., et al. (2021). Limitation of phosphate assimilation maintains cytoplasmic magnesium homeostasis. Proc Natl Acad Sci U S A.118 (11), e2021370118. [PubMed]
  2. Kwong, J. Q., et al. (2021). Mitochondrial functional resilience after TFAM ablation in the adult heart. Am J Physiol Cell Physiol.320 (6), C929-C942. [PubMed]
  3. Coates, H. W. et al. (2021). The mammalian cholesterol synthesis enzyme squalene monooxygenase is proteasomally truncated to a constitutively active form. J Biol Chem., Online ahead of print. [PubMed]

Applicable patents and legal notices are available at legal notices.

How do I Request a Quote?

To request a quote for products:

  1.  Log in to VectorLabs.com
  2.  Add the items you wish to quote to the cart;
  3.  View your cart;
  4.  Click on the “Request a Quote” button found below the items in your cart;
  5.  Download your quote