Vector Laboratories is closed for the President’s Day on Monday, February 19th. We will be back in the office on Tuesday, February 20th.
We will respond to emails upon our return. Have a wonderful day.

Click-&-Go™ Lys-to-Lys Protein-Protein Conjugation Kit (CCT-1008)

[text-backorder]

Description

The Lys-to-Lys Protein-Protein Conjugation Kit (Cat. # 1008) provides a sufficient amount of reagents to perform two protein-protein conjugation reactions. Any two lysine containing proteins (100-500 µg) in a volume of 100 µL (1-5 mg/mL) can be efficiently conjugated in less than 3 hours, start-to-finish.

The Lys-to-Lys Protein-Protein Conjugation kit provides all of the necessary reagents to perform two protein-protein conjugation reactions. Conjugates are formed by the very efficient, catalyst-free bio-orthogonal ligation reaction between trans-cyclooctene (TCO) and tetrazine (Tz) functional groups. In the protocol provided, two proteins (or any other amine-containing biopolymers) are labeled separately with TCO and Tetrazine reagents. The labeled molecules are then mixed for 60 minutes in any suitable buffer to form a conjugate.

The extremely fast kinetics of the TCO/Tz ligation reaction enables rapid conjugation (30-60 min) of lysine containing proteins to each other at low concentrations (e.g. 5-10 µM), with >99% conversion of the limiting protein in mild buffered media (e.g. PBS pH 7.5). Another benefit is the long-term stability of TCO and Tz functional groups on modified proteins stored in aqueous buffered media (e.g. maintaining > 90% reactivity after 1 month at 4oC, pH 7.5). This stability allows for worry-free conjugation results without the sensitive timing of reactions associated with classical thiol/maleimide chemistry.

1008 schamtic workflow
Figure 1 Schematic representation of protein protein conjugation

Specifications

Unit Size1 kit

Number of Reactions

2
Storage Conditions4C
Shipping ConditionsAmbient temperature

Protein Labeling Calculator

[protein-labeling-calculator]

Applicable patents and legal notices are available at legal notices.

How do I Request a Quote?

To request a quote for products:

  1.  Log in to VectorLabs.com
  2.  Add the items you wish to quote to the cart;
  3.  View your cart;
  4.  Click on the “Request a Quote” button found below the items in your cart;
  5.  Download your quote