Bis-dPEG®₅-NHS ester (QBD-10224)

Description

Bis-dPEG®5-NHS ester, product number QBD-10224, is a homobifunctional, amine-reactive, single molecular weight PEG crosslinker with a short, discrete PEG (dPEG®) chain length. Each end of the chain terminates as the N-hydroxysuccinimidyl (NHS) ester of propionic acid. Unlike traditional hydrophobic crosslinkers, Bis-dPEG®5-NHS ester will not cause problems such as aggregation, precipitation, and inactivation when conjugated to biomolecules. Various uses for Bis-dPEG®5-NHS ester have been published in the scientific literature.

NHS esters react with free amines such as the ε-amines of lysine. The optimal pH range for NHS esters to react with free amines is 7.0 – 7.5. However, NHS esters can react with free amines with pH as low as 6.5. NHS esters are susceptible to hydrolysis in aqueous media. As the pH increases, the hydrolysis rate of the ester increases. Thus, we strongly discourage storing Bis-dPEG®5-NHS ester in water or aqueous buffer. Instead, we recommend that customers make new solutions of the product as needed, use them immediately, and discard unused solutions after use. If customers desire to store the product in solution, we recommend the use of a pure, anhydrous, water-miscible solvent such as dimethyl sulfoxide (DMSO), N,N-dimethylacetamide (DMAC), or N,N-dimethylformamide (DMF). DMSO, DMAC, or DMF can be dried chemically or by storing for a minimum of 24 hours over 3 Å molecular sieves. With DMF, use only fresh solvent as the compound decomposes over time to form free amines that will react with the NHS esters.

Specifications

Documents

References

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.

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G-Quadruplex Induction by the Hairpin Pyrrole–Imidazole Polyamide Dimer. Shunsuke Obata, Sefan Asamitsu, Kaori Hashiya, Toshikazu Bando, and Hiroshi Sugiyama. Biochemistry. 2018, 57, pp 498-502. December 13, 2017. DOI: 10.1021/acs.biochem.7b01059.

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Toward the Development of GE11-Based Radioligands for Imaging of Epidermal Growth Factor Receptor-Positive Tumors. Benedikt Judmann, Diana Braun, Ralf Schirrmacher, Björn Wängler, Gert Fricker, and Carmen Wängler. ACS Omega 2022, 7, 31, 27690–27702. 2022. https://doi.org/10.1021/acsomega.2c03407

Multivalent Benzamidine Molecules for Plasmin Inhibition: Effect of Valency and Linker Length. Tanmaye Nallan Chakravarthula, Dr. Ziqian Zeng, Prof. Nathan J. Alves. ChemMedChem, Volume 17, Issue 22, (2022), 09/16/2022, https://doi.org/10.1002/cmdc.202200364.