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Streptavidin, Texas Red™

SA-5006-1
SKU Unit Size Price Qty
SA-5006-1 1 mg
$182.00
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Description

Texas Red Streptavidin is a highly fluorescent conjugate of streptavidin and sulforhodamine 101. It can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry.

Features:

  • Highly purified and possesses very low non-specific binding properties
  • Has a high fluorochrome to protein ratio
  • Compared to conventional primary and secondary fluorescent techniques, can provide greater sensitivity and lower background staining
  • Excitation maximum: 595 nm
  • Emission maximum: 615 nm
  • Color:  Red

Specifications

More Information
Unit Size 1 mg
Applications Immunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation
Recommended Usage The recommended concentration range for use is 5-30 µg/ml.
Solution 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide
Maximum Excitation 595-604 nm
Maximum Emission 606-615 nm
Recommended Storage 2-8 °C
Concentration 1.0 mg/ml active conjugate
Conjugate Texas Red
Color of Fluorescence Red
Format Concentrate

Documents

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Prostate: Prostate Specific Antigen (rp), fluorescein labeled using the ProtOn Fluorescein Labeling Kit. Propidium Iodide counterstain (red). Mounted in VECTASHIELD HardSet Mounting Medium. VECTASHIELD® HardSet™ Antifade Mounting Medium Catalog #: H-1400-10
Astrocytes: Stained for GFAP and detected with Dylight 488 labeled secondary antibody. Mounted in VECTASHIELD HardSet Mounting Medium with DAPI. Image courtesy of Dr Emma East, Department of Life Sciences, The Open University, U.K.* VECTASHIELD® HardSet™ Antifade Mounting Medium with DAPI Catalog #: H-1500-10

Citations

Technical Information

Vector Laboratories fluorochrome-conjugated streptavidin and avidin reagents are highly purified and possess very low non-specific binding properties. They have extremely high affinity for biotin. Amplification of fluorescent signals can be easily achieved with our biotinylated secondary antibodies followed by our highly purified fluorochrome-labeled streptavidin or avidin. Using a biotin/avidin or biotin/streptavidin detection system results in an additional layer of amplification over a directly conjugated secondary antibody.

Since the excitation and emission maxima are well separated from those of fluorescein, Texas Red Streptavidin can be employed with Fluorescein Streptavidin or other fluorescein conjugates to simultaneously localize two antigens in the same tissue section. Texas Red Streptavidin is also ideal for flow cytometry using instruments equipped with dye lasers.

Home All Products Fluorophore Conjugated Avidin/Streptavidin Streptavidin, Texas Red™