AMCA Streptavidin is produced by conjugating streptavidin with a coumarin fluorescent dye, 7-amino-4-methylcoumarin-3-acetic acid. This derivative excites in the ultraviolet (350 nm) and emits in the visible (450 nm) producing an intense blue fluorescence.
Amplification of fluorescent signals can be easily achieved with our biotinylated secondary antibodies followed by our highly purified fluorochrome-labeled streptavidin or avidin. Using a biotin/avidin or biotin/streptavidin detection system results in an additional layer of amplification over a directly conjugated secondary antibody.
Features:
| Unit Size | 1 mg |
|---|---|
| Applications | Immunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation |
| Recommended Usage | For diluting this product, we recommend a HEPES- or biocarbonate-buffered saline solution, approximately pH 8.2.Avoid using RPMI 1640 or other biotin-containing solutions as diluents.Serum also can contain biotin and should not be added to diluents. The recommended concentration range for use is 10-30 µg/ml. |
| Solution | 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide |
| Maximum Excitation | 345-355 nm |
| Maximum Emission | 448-454 nm |
| Recommended Storage | 2-8 °C |
| Concentration | 1.0 mg/ml |
| Conjugate | AMCA |
| Color of Fluorescence | Blue |
| Format | Concentrate |
Streptavidin/Biotin Blocking Kit
Catalog #:
SP-2002
Goat Anti-Streptavidin Antibody, Biotinylated
Catalog #:
BA-0500-.5
VECTASHIELD® Antifade Mounting Medium
Catalog #:
H-1000-10
VECTASHIELD® Antifade Mounting Medium with Propidium Iodide (PI)
Catalog #:
H-1300-10
VECTASHIELD® HardSet™ Antifade Mounting Medium
Catalog #:
H-1400-10
VECTASHIELD® HardSet™ Antifade Mounting Medium with Phalloidin
Catalog #:
H-1600-10
Vector Laboratories fluorochrome-conjugated streptavidin and avidin reagents are highly purified and possess very low non-specific binding properties. They have extremely high affinity for biotin. These fluorescent conjugates can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry.
Most paraffin-embedded tissues have little autofluorescence under those conditions. AMCA Streptavidin may provide an advantage in many labeling situations over conjugates of fluorescein and related dyes. AMCA Streptavidin can be used on frozen or paraffin-embedded sections as a single label or in combination with other fluorescent conjugates for multiple labeling studies.
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