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5-TAMRA cadaverine can used to modify carboxylic acid group in the presence of activators (e.g. EDC, or DCC) or activated esters (e.g. NHS esters) through a stable amide bond. It also can be reversibly coupled to aldehydes and ketones to form a Schiff base – which can be reduced to a generate stable amine derivative by sodium borohydride (NaBH4) or sodium cyanoborohydride (NaCNH3).
Although the mixed isomers of 5(6)-TAMRA cadaverine is a preferred, routinely used orange-fluorescent dye for staining proteins, it is rearly used for labeling peptides and nucleotides. Purification of 5(6)-TAMRA labeled peptide and nucleotides might be troublesome due to significant signal broadening in HPLC purification. Peptides and nucleotides labeled with a single isomer TAMRA usually give better resolution in HPLC purification that is often required in the conjugation processes.
Unit Size | 5 mg, 25 mg, 100 mg, 1000 mg |
---|---|
Reactivity | Primary amines |
Abs/Em Maxima | 553/575 nm |
Extinction coefficient | 91,000 cm-1M-1 |
Solubility | DMSO, DMF, MeOH |
Spectrally similar dyes | Alexa Fluor® 546, TAMRA, CF™ 543, MB™ 543 |
Molecular weight | 627.24 (TFA salt) |
Storage Conditions | -20°C. |
Shipping Conditions | Ambient temperature |
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