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5-TAMRA Alkyne (CCT-1255)

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5-TAMRA Alkyne (also known as TAMRA Alkyne, isomer 5) is the red-fluorescent probe that is compatible with various excitation sources including mercury arc, tungsten and xenon arc lamps, the 544 nm line of the Helium-Neon laser and the 532 nm green laser line. It is predominantly used for detection of terminal azide-tagged biomolecules via a copper-catalyzed click reaction (CuAAC).

Description

5-TAMRA Alkyne (also known as TAMRA Alkyne, isomer 5) is the red-fluorescent probe that is compatible with various excitation sources including mercury arc, tungsten and xenon arc lamps, the 544 nm line of the Helium-Neon laser and the 532 nm green laser line. It is predominantly used for detection of terminal alkyne-tagged biomolecules via a copper-catalyzed click reaction (CUAAC). It also reacts with strained cyclooctyne via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures.

Although the mixed isomers of 5(6)-TAMRA Alkyne is a preferred, routinely used red fluorescent dye for staining proteins, labeling peptides and nucleotides with TAMRA mixed isomers might be troublesome due to significant signal broadening in HPLC purification. Peptides and nucleotides labeled with a single isomer TAMRA usually give better resolution in HPLC purification that is often required in the conjugation processes.

5-TAMRA Alkyne is structurally identical to Tetramethylrhodamine (TAMRA) Alkyne (5-Carboxytetramethylrhodamine, Propargylamide), 5-isomer ,Catalog number: T10183.

Specifications

Unit Size1 mg, 5 mg, 25 mg, 100 mg
Abs/Em Maxima553/575 nm
Extinction Coefficient
89,000
Spectrally Similar DyesAlexa Fluor® 546, Atto™ 543, CF® 543 Dye
Molecular weight467.52
CASN/A
SolubilityDMSO, DMF
Purity>95% (HPLC)
AppearanceDark red amorphous solid
Storage Conditions-20°C. Desiccate
Shipping ConditionsAmbient temperature

Abs/Em Spectra

TAMRA TAMRA

Selected References

  1. Kang, D., et al. (2021). Bioorthogonal Retro-Cope Elimination Reaction of N, N-Dialkylhydroxylamines and Strained Alkynes. J Am Chem Soc.143 (15), 5616-5621. [PubMed]
  2. Ranzinger, R., et al. (2020). Mass Spectrometric Method for the Unambiguous Profiling of Cellular Dynamic Glycosylation. ACS Chem. Biol., 15, 10, 2692-2701. [ACSPublications]

Applicable patents and legal notices are available at legal notices.

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