ABC (Avidin-Biotin Complex) Kits

Time-tested, versatile avidin-biotin complex kits (ABC kits) for immunodetection

With more than 60,000 citations to its credit, Vector Labs's VECTASTAIN^® Avidin-Biotin Complex Staining Kits (ABC Kits) exploit the high-affinity avidin-biotin interaction for reliable, highly sensitive detection of any biotinylated molecule.

Use these kits to visualize biotinylated antibodies, lectins, neuronal tracers, nucleic acids, and ligands in applications such as:

• Immunohistochemistry (IHC) and immunocytochemistry (ICC)
• Protein and nucleic acid blot detection
• In situ hybridization detection (ISH)
• Enzyme immunoassays (ELISAs)
• Neuronal tracing 

Use the VECTASTAIN ABC system if you are looking for:

• High sensitivity with low background — due to the form and number of active enzyme molecules associated with the avidin/biotinylated enzyme complex
• Versatility — detect any molecule that is biotinylated in a wide variety of applications and used in a number of tissue types and species
• Modularity —  configure for compatibility with a large number of different primary antibodies and target species, and choose among a selection of detection enzymes
• Convenience and economy — use the avidin-biotin enzyme complexes for several days after preparation 

VECTASTAIN ABC systems are widely popular and are a staple product for immunodetection. They are available with horseradish peroxidase (HRP) or alkaline phosphatase (AP) enzyme detection.

• The HRP kits produce sharp, dense precipitates with crisp localization and are a preferred choice for many applications
• The AP kits are ideal when working with tissues with high endogenous peroxidase activity

Both systems are available without (standard kit) or with biotinylated secondary antibodies.