ABC (Avidin-Biotin Complex) Kits

Time-tested, versatile avidin-biotin complex kits (ABC kits) for immunodetection

With more than 60,000 citations to its credit, Vector Labs's VECTASTAIN® Avidin-Biotin Complex Staining Kits (ABC Kits) exploit the high-affinity avidin-biotin interaction for reliable, highly sensitive detection of any biotinylated molecule.

Use these kits to visualize biotinylated antibodies, lectins, neuronal tracers, nucleic acids, and ligands in applications such as:

  • Immunohistochemistry (IHC) and immunocytochemistry (ICC)
  • Protein and nucleic acid blot detection
  • In situ hybridization detection (ISH)
  • Enzyme immunoassays (ELISAs)
  • Neuronal tracing 

Use the VECTASTAIN ABC system if you are looking for:

  • High sensitivity with low background — due to the form and number of active enzyme molecules associated with the avidin/biotinylated enzyme complex
  • Versatility — detect any molecule that is biotinylated in a wide variety of applications and used in a number of tissue types and species
  • Modularity —  configure for compatibility with a large number of different primary antibodies and target species, and choose among a selection of detection enzymes
  • Convenience and economy — use the avidin-biotin enzyme complexes for several days after preparation 

VECTASTAIN ABC systems are widely popular and are a staple product for immunodetection. They are available with horseradish peroxidase (HRP) or alkaline phosphatase (AP) enzyme detection.

  • The HRP kits produce sharp, dense precipitates with crisp localization and are a preferred choice for many applications
  • The AP kits are ideal when working with tissues with high endogenous peroxidase activity

Both systems are available without (standard kit) or with biotinylated secondary antibodies.


Explore VECTASTAIN ABC (Avidin-Biotin Complex Kits)

VECTASTAIN ABC HRP systems deliver enhanced signal sensitivity with low background interference. They are compatible with a wide range of specimens and are a preferred choice for applications such as IHC that require sharp, distinct localization of the target antigen.

The VECTASTAIN ABC-AmP kit is an amplified ABC alkaline phosphatase reagent for the detection of mouse or rabbit primary antibodies on nitrocellulose or PVDF membranes.

VECTASTAIN® ABC-AP (alkaline phosphatase) Kits provide high sensitivity avidin-biotin based detection in immunohistochemistry (IHC), immunocytochemistry (ICC), protein and nucleic acid blot, in situ hybridization (ISH), and enzyme immunoassay (ELISA) detection. The VECTASTAIN® ABC-AP Kits are ideally suited for specimens with high endogenous peroxidase activity or to introduce substrate color choices for multiple antigen labeling (multiplexing).


Resources

IHC Troubleshooting Guide for ABC Systems

A quick reference guide that outlines the systematic controls to help identify background sources in an immunohistochemistry protocol using ABC systems.  Also troubleshoot issues of weak or no staining. Laminated.

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