ABC (Avidin-Biotin Complex) Kits

Time-tested, versatile avidin-biotin complex kits (ABC kits) for immunodetection

With more than 60,000 citations to its credit, Vector Labs's VECTASTAIN® Avidin-Biotin Complex Staining Kits (ABC Kits) exploit the high-affinity avidin-biotin interaction for reliable, highly sensitive detection of any biotinylated molecule.

Use these kits to visualize biotinylated antibodies, lectins, neuronal tracers, nucleic acids, and ligands in applications such as:

  • Immunohistochemistry (IHC) and immunocytochemistry (ICC)
  • Protein and nucleic acid blot detection
  • In situ hybridization detection (ISH)
  • Enzyme immunoassays (ELISAs)
  • Neuronal tracing 

Use the VECTASTAIN® ABC system if you are looking for:

  • High sensitivity with low background — due to the form and number of active enzyme molecules associated with the avidin/biotinylated enzyme complex
  • Versatility — detect any molecule that is biotinylated in a wide variety of applications and used in a number of tissue types and species
  • Modularity —  configure for compatibility with a large number of different primary antibodies and target species, and choose among a selection of detection enzymes
  • Convenience and economy — use the avidin-biotin enzyme complexes for several days after preparation 

VECTASTAIN® ABC systems are widely popular and are a staple product for immunodetection. They are available with horseradish peroxidase (HRP) or alkaline phosphatase (AP) enzyme detection.

  • The HRP kits produce sharp, dense precipitates with crisp localization and are a preferred choice for many applications
  • The AP kits are ideal when working with tissues with high endogenous peroxidase activity

Both systems are available without (standard kit) or with biotinylated secondary antibodies.