Your path to simplified multiplexing starts with VectaPlex™. This kit is designed to remove antibodies and other non-covalently bound detection reagents from FFPE tissue sections in immunohistochemistry (IHC), immunofluorescence (IF) and tyramide signal amplification (TSA) workflows.
Benefits include:
The first image above is a composite image generated from individual images taken during six rounds of immunofluorescence staining using VectaPlex. FFPE stomach; six mouse primary antibodies – CD20 (red), CD34 (yellow), Desmin (cyan), AE1/AE3 (magenta), CD3 (gray), Vimentin (green) – detected with DyLight™ 488 horse anti-mouse IgG. Blue indicates DAPI stain. Vector® TrueView® Autofluorescence Quencher Kit applied to suppress background fluorescence.
The second image above is a 4-plex IHC image taken of esophagus tissue stained for CD68 (brown) using ImmPACT DAB, CD20 (red) using ImmPACT Vector Red, CD34 (blue) using Vector Blue, and Desmin (grey) using ImmPACT SG. Each round of staining was performed using normal IHC protocols with VectaPlex treatment between each round of staining.
The third image above was captured after three rounds of tyramide staining. VectaPlex was used after each round. FFPE tonsil is shown, following application of 3 mouse primary antibodies – CD3 (red), CD68 (green) and AE1/AE3 (purple) – detected using Opal 3-plex TSA Manual Detection Kit. Blue indicates DAPI stain.
Unit Size | 125 reactions / kit |
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Applications | Immunohistochemistry, Immunofluorescence, Tyramide Signal Amplification |
Target Species | Universal |
Recommended Storage | (15–25° C) |
Format | Ready-to-Use |
This ready-to-use kit is designed to remove antibodies and other non-covalently bound detection reagents from formalin-fixed paraffin-embedded (FFPE) tissue sections in immunohistochemistry, immunofluorescence or tyramide signal amplification workflows.
VectaPlex simplifies sample processing for manual workflows, accelerates multiplex assay optimization, preserves precious sample, and maintains tissue integrity and morphology through multiple cycles of antibody binding and removal.
Applicable patents and legal notices are available at legal notices.
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