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Rabbit Anti-Goat IgG Antibody (H+L), Unconjugated (AI-5000-1.5)

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Description

Unconjugated Rabbit Anti-Goat IgG Antibody binds to goat primary antibodies for immunohistochemistry and many other applications. It is also suitable for use with sheep and bovine IgG primary antibodies

Features:

  • Ultrapure, high affinity antibody 
  • Thoroughly adsorbed against serum and immunoglobulins from potentially interfering species
  • Recognizes both heavy and light chains (H+L)
  • Ready for iodination, fluorochrome labeling, or enzyme conjugations
  • Can be employed as a capture antibody in enzyme immunoassays or in other assays requiring carrier-free immunoglobulins

Specifications

Unit Size1.5 mg
ApplicationsImmunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot, ELISAs
Concentration3 mg/ml
Recommended Storage2-8 °C; Store frozen for long term storage
Solution10 mM sodium phosphate, pH 7.8, 0.15 M NaCl, 0.08% sodium azide, 20 mg/ml sucrose
Target SpeciesGoat
ConjugateUnconjugated
Host SpeciesRabbit
FormatConcentrate

Citations

Technical Information

The rabbit anti-goat IgG antibodies are prepared by hyperimmunizing animals in a manner that produces high affinity antibodies. These are then purified by an affinity chromatography procedure designed to remove any low affinity antibodies which may be present. Cross-reactivities that are likely to interfere with specific labeling are removed by solid-phase adsorption techniques. The final product is then subjected to rigorous quality control assays including immunodiffusion, solid-phase enzyme immunoassays, gel electrophoresis and solid-phase binding assays. In preparing the labeled antibodies, great care is taken to ensure the maximum degree of labeling with no alteration in the specificity and affinity of the antibody. The labeled antibody then undergoes a further series of quality control assays, including immunohistochemical analysis. Unless otherwise specified, our antibodies will recognize both heavy and light chains (H+L).

Unconjugated Rabbit Anti-Goat IgG (H+L) Antibody is supplied in solution.

As well as recognizing goat IgG, this antibody is suitable to detect sheep and bovine IgG primary antibodies.

The use of bovine products such as casein, serum, albumin or non-fat dry milk as blocking agents in ELISA or blot assays may produce a high background due to cross-reactivity of this antibody with bovine immunoglobulins that may be present. If this antibody is to be used in these applications, blocking with Animal-Free Blocker® solution (Cat. No. SP-5030) is recommended.

Applicable patents and legal notices are available at legal notices.

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