SY-9 NHS ester (FP-1706)

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Description

SY-9 (QSY®-9 equivalent) is a more hydrophilic version of the popular SY-7 dark quencher (QSY®-7 equivalent). This nonfluorescent acceptor dye is often used for the preparation of peptide FRET probes. A broad and intense quenching range from 500-600 nm makes it useful as an acceptor in fluorescence resonance energy transfer (FRET) applications in conjunction with dyes emitting at 500 nm to 600 nm, such as Fluorescein, Oregon Green, Carboxyrhodamine 110 (Rhodamine Green), Alexa Fluor® 488, 532, 546, 555, 568, Cy3, TAMRA and ROX dyes.

The NHS ester (or succinimidyl ester) is the most popular amine reactive group for labeling with the primary amines of proteins (Lys), amine-modified oligonucleotides, and other amine-containing molecules. The labeling occurs most efficiently at pH 7-9 and forms a stable, covalent amide bond.

Specifications

Unit Size5 mg, 25 mg, 100 mg
Reactivity
Primary amine
Abs/Em Maxima560 nm
Extinction coefficient85,000 cm-1M-1
SolubilityDMSO, DMF
Spectrally similar dyesCarboxyrhodamine 110 (Rhodamine Green), Alexa Fluor® 488, 532, 546, 555, 568, Cy3, TAMRA and ROX
Molecular weight914.97
Storage Conditions-20°C.
Shipping ConditionsAmbient temperature

Abs/Em Spectra

Sy 7 9 Sy-7_9 Vectorlabs

Protein Labeling Calculator

Enter only one — either volume or amount of protein to be labeled
Enter only one — either maximum concentration of DMSO or volume DMSO added to label

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