NHS-dPEG®24-biotin, product number QBD-10774, is an amine-reactive biotinylation product containing a single molecular weight, discrete PEG (dPEG®) linker that is 76 atoms long. Although biotin usually is poorly soluble in water, the amphiphilic dPEG® linker creates excellent water solubility while maintaining organic solvent solubility. The N-hydroxysuccinimidyl (NHS) moiety of NHS-dPEG®24-biotin reacts optimally with free primary amines in the pH range 7.0 – 7.5., forming stable amide bonds. Unlike the classic, hydrophobic biotin linkers, LC-biotin and LC-LC-biotin, NHS-PEG®24-biotin does not cause protein aggregation due to over-labeling of the target molecule. Labeling experiments, supramolecular construction, affinity chromatography, pull-down assays, the creation of biotinylated antibodies, and many other applications can use NHS-dPEG®24-biotin to take advantage of the exceptionally high affinity between biotin and avidin or streptavidin.
| Unit Size | 50mg, 1000mg |
|---|---|
| Molecular Weight | 1469.72; single compound |
| Chemical formula | C₆₅H₁₂₀N₄O₃₀S |
| CAS | 365441-71-0 |
| Purity | > 98% |
| Spacers | dPEG® Spacer is 76 atoms and 97.7 Å |
| Shipping | Ambient |
| Typical solubility properties (for additional information contact Customer Support) | Methylene chloride, Acetonitrile, DMAC or DMSO. |
| Storage and handling | -20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure. |
Greg T. Hermanson, Bioconjugate Techniques, 2nd Edition, Elsevier Inc., Burlington, MA 01803, April, 2008 (ISBN-13: 978-0-12-370501-3; ISBN-10: 0-12-370501-0). Specifically see pp. 726-730 in his chapter 18 on discrete PEG compounds for pegylation applications.
Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.
Visualizing mechanical tension across membrane receptors with a fluorescent sensor. Daniel R Stabley, Carol Jurchenko, Stephen S Marshall & Khalid S Salaita. Nature Methods. 2011, 9, PP 64–67. October 30, 2011. doi:10.1038/nmeth.1747.
Hepatocyte Targeting of Nucleic Acid Complexes and Liposomes by a T7 Phage p17 Peptide. So C. Wong, Darren Wakefield, Jason Klein, Sean D. Monahan, David B. Rozema, David L. Lewis, Lori Higgs, James Ludtke, Alex V. Sokoloff, and Jon A. Wolff. Mol. Pharm. 2006, 3, (4) pp 386-397. March 28, 2006. DOI: 10.1021/mp050108r.
Visualizing mechanical tension across membrane receptors with a fluorescent sensor. Daniel R Stabley, Carol Jurchenko, Stephen S Marshall & Khalid S Salaita. Nature Method. 2012, 9 (1) pp 64-67. October 30, 2011. DOI:10.1038/nmeth.1747
Applicable patents and legal notices are available at legal notices.
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