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MB™ 488 is a very hydrophilic, water-soluble green emitting dye. Structurally related to Alexa Fluor® 488 dye, MB™ 488 dye is a next step in improving water solubility, brightness and minimizing self-quenching. Characteristic features of the label are strong absorption, high fluorescence quantum yield, and very high photostability. MB™ 488 is a highly suitable dye for single-molecule detection applications and high-resolution microscopy such as PALM, dSTORM, STED etc. Additionally MB™ 488 is an excellent dye for flow cytometry (FACS), fluorescence in-situ hybridization (FISH) and many other applications.
The NHS Ester reacts specifically and efficiently with a primary amine (e.g., side chain of lysine residues or aminosilane-coated surfaces) at pH 7-9 to form a stable, covalent amide bond. The NHS ester (or succinimidyl ester) is the most popular tool for conjugating dyes to the primary amines of protein or antibody (Lys), amine-modified oligonucleotides, and other amine-containing molecules.
Unit Size | 1 mg, 5 mg, 25 mg, 100 mg |
---|---|
Reactivity | Primary amine |
Abs/Em Maxima | 501/524 nm |
Extinction coefficient | 86,000 cm-1M-1 |
Solubility | Water, DMSO, DMF |
Spectrally similar dyes | Alexa Fluor® 488, DyLight® 488, Fluorescein, Oregon Green 488 |
Molecular weight | 752.69 |
Storage Conditions | -20°C. |
Shipping Conditions | Ambient temperature |
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