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Enzyme Conjugated Secondary Antibodies

AP conjugated secondary antibodies (anti-rabbit IgG) used to detect ki67 and Cox-2Our high affinity antibodies are purified by affinity chromatography and adsorbed to remove any cross-reactivities that are likely to interfere with specific labeling. These antibodies are then cross-linked with the highest specific activity alkaline phosphatase (AP) or horseradish peroxidase (HRP). A proprietary procedure for conjugation allows the maximum preservation of enzyme activity and antibody specificity. These HRP conjugated secondary antibodies and AP conjugated secondary antibodies are finally subjected to rigorous quality control assays.  

Due to rigorous manufacturing protocols and testing procedures, the HRP conjugated secondary antibody and the AP conjugated antibody can be used in a variety of applications including tissue and cell staining, ELISAs, and blots.

For maximum sensitivity and convenience, the one-step ImmPRESS™ Peroxidase Polymer Systems can substitute for HRP or AP conjugated secondary antibodies in immunohistochemistry and immunocytochemistry applications.

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