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Griffonia Simplicifolia Lectin I (GSL I) Isolectin B4, Unconjugated

L-1104-1
SKU Unit Size Price Qty
L-1104-1 1 mg
$240.00
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Description

GSL I-B4 isolectin contains only the B subunits. It is a useful marker for endothelial cells from nonprimates such as mouse, rat, rabbit, and goat as well as a marker for non-peptidergic unmyelinated primary afferent neurons. This “B”-rich lectin preferentially agglutinates blood group B cells and is specific for α-galactose residues.

Specifications

More Information
Unit Size 1 mg
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Glycobiology
Recommended Usage Although many buffers can be employed for reconstituting and diluting this lectin, 10 mM HEPES buffered saline, pH 8.5, 0.1 mM CaCl2 is recommended.For preserving solutions, stored at 4 ºC, 0.08% sodium azide can be used.
Recommended Storage 2-8 °C
Conjugate Unconjugated
Sugar Specificity Galactose

Documents

Related Products

Galactose Galactose Catalog #: S-9003
Griffonia Simplicifolia Lectin I (GSL I) Isolectin B4, Fluorescein Griffonia Simplicifolia Lectin I (GSL I) Isolectin B4, Fluorescein Catalog #: FL-1201-.5
Mouse tongue (formalin-fixed, paraffin-embedded) stained with DyLight 594-Labeled GSL 1, Isolectin B4 (red) and counterstained with DAPI (blue). Griffonia Simplicifolia Lectin I (GSL I) Isolectin B4, DyLight™ 594 Catalog #: DL-1207-.5
Griffonia Simplicifolia Lectin I (GSL I) Isolectin B4, Biotinylated Griffonia Simplicifolia Lectin I (GSL I) Isolectin B4, Biotinylated Catalog #: B-1205-.5

Citations

Technical Information

GSL I is a family of glycoproteins with molecular weights of approximately 114 kDa. There are two types of subunits, termed A and B, with slightly different molecular weights. These subunits combine to form tetrameric structures, resulting in five isolectins. The A -rich lectin preferentially agglutinates blood group A erythrocytes and thus appears to be specific for α-N-acetylgalactosamine residues, while the B -rich lectin preferentially agglutinates blood group B cells and is specific for α-galactose residues. Our GSL I is a mixture of the five isolectins. GSL I has been reported to bind several glycoproteins including laminin.


Inhibiting Sugar: 500 mM galactose or 100 mM raffinose
 
Home Griffonia simplicifolia I isolectin B4 (GSL I IB4) Griffonia Simplicifolia Lectin I (GSL I) Isolectin B4, Unconjugated