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Lycopersicon esculentum (Tomato) Lectin (LEL, TL), Unconjugated

L-1170-2
SKU Unit Size Price Qty
L-1170-2 2 mg
$186.00
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Description

Tomato lectin (from Lycopersicon esculentum) is an effective marker of blood vessels and microglial cells in rodents. Conjugation of the lectin with a fluorophore facilitates fast, one-step detection and visualization using intravascular perfusion methods or direct application to tissue sections.

Specifications

More Information
Unit Size 2 mg
Applications Glycobiology
Recommended Usage Although many buffers can be employed for reconstituting this lectin, 10 mM HEPES buffered saline, pH 8.5, 0.1 mM CaCl2 is recommended.
Recommended Storage 2-8 °C; for long term storage, aliquots may be stored frozen or preserved with 0.08% sodium azide in the recommended buffer and stored at 2-8 °C
Conjugate Unconjugated
Sugar Specificity [GlcNAc]1-3, N-Acetylglucosamine

Documents

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Chitin Hydrolysate Chitin Hydrolysate Catalog #: SP-0090-10
Lycopersicon Esculentum (Tomato) Lectin (LEL, TL), Texas Red™ Lycopersicon Esculentum (Tomato) Lectin (LEL, TL), Texas Red™ Catalog #: TL-1176-1
Retina from mice, perfused with DyLight 594-LEL. The retina were sectioned and mounted in Vectashield HardSet+DAPI. Image courtesy of George W. Smith, Florida Atlantic University. Lycopersicon Esculentum (Tomato) Lectin (LEL, TL), DyLight™ 594 Catalog #: DL-1177-1
Mouse kidney, formalin-fixed, paraffin-embedded: DyLight 488 Labeled Lycopersicon Esculentum lectin (green) mounted in VECTASHIELD HardSet with DAPI (blue). Lycopersicon Esculentum (Tomato) Lectin (LEL, TL), DyLight™ 488 Catalog #: DL-1174-1
Frozen section of colon viewed under fluorescein filter: Lycopersicon esculentum lectin (orange staining with Vector Red) and glandular epithelium (green fluorescein label). Lycopersicon esculentum (Tomato) Lectin (LEL, TL), Biotinylated Catalog #: B-1175-1

Citations

Technical Information

Tomato lectin (from Lycopersicon esculentum) is a very stable single subunit glycoprotein containing about 50 percent arabinose and galactose and may form multimeric aggregates in solution. Tomato lectin, although sharing some specificities with potato lectin, Datura lectin, and wheat germ agglutinin, has been reported to be dissimilar in many respects. LEL binds well to glycophorin and Tamm-Horsfall glycoprotein and has been used effectively to label vascular endothelium in rodents.

Inhibiting/Eluting Sugar: Chitin Hydrolysate

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