This lectin consists of two subunits of 60 kDa which can be dissociated by reducing agents into closely related chains between 27 kDa and 33 kDa. One of the chains appears to be common to the “B” chain of another castor bean lectin, ricin, while the other chain is unique to RCA I. The B chain binds to galactose or N-acetylgalactosamine residues of membrane glycoconjugates.
Fluorescein labeled Ricinus communis agglutinin I has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes. The excitation maximum is at 495 nm and the emission maximum is at 515 nm.
| Unit Size | 5 mg |
|---|---|
| Applications | Immunofluorescence, Glycobiology |
| Recommended Usage | The recommended concentration range for use is 5-20 µg/ml. |
| Recommended Storage | 2-8 °C |
| Maximum Excitation | 495-500 nm |
| Inhibiting and/or Eluting Sugar | 200 mM galactose (S-9003) or lactose (S-9004) |
| Maximum Emission | 514-521 nm |
| Solution | 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2, 5 mM lactose |
| Concentration | 5 mg active conjugate/ml |
| Conjugate | Fluorescein |
| Color of Fluorescence | Green |
| Sugar Specificity | Galactose, Lactose |
Ricinus Communis Agglutinin I (RCA I, RCA120), Unconjugated
Catalog #:
L-1080-10
Ricinus Communis Agglutinin I (RCA I, RCA120), Rhodamine
Catalog #:
RL-1082-5
Ricinus Communis Agglutinin I (RCA I, RCA120), Biotinylated
Catalog #:
B-1085-5
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