GSL I is a family of glycoproteins with molecular weights of approximately 114 kDa. There are two types of subunits, termed “A” and “B”, with slightly different molecular weights. These subunits combine to form tetrameric structures, resulting in five isolectins. The “A”-rich lectin preferentially agglutinates blood group A erythrocytes and thus appears to be specific for α-N-acetylgalactosamine residues, while the “B”-rich lectin preferentially agglutinates blood group B cells and is specific for α-galactose residues. Our GSL I is a mixture of the five isolectins. GSL I has been reported to bind several glycoproteins including laminin. Biotinylated GSL I has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin.
| Unit Size | 2 mg |
|---|---|
| Applications | Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs |
| Recommended Usage | For most applications we recommend a freshly prepared working solution of 5-20 µg/ml in the above buffer. |
| Recommended Storage | 2-8 °C; Store frozen for long term storage |
| Inhibiting and/or Eluting Sugar | Inhibiting/Eluting Sugar: Mixture of 200 mM galactose (S-9003)/200 mM N-acetylgalactosamine (S-9001). |
| Solution | 10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% sodium azide, 0.1 mM CaCl2. |
| Concentration | 2 mg active conjugate/ml |
| Conjugate | Biotinylated |
| Sugar Specificity | Galactose, N-Acetylgalactosamine |
Glycoprotein Eluting Solution for Galactose/GalNAc Binding Lectins
Catalog #:
ES-2100-100
N-acetylgalactosamine
Catalog #:
S-9001
Galactose
Catalog #:
S-9003
Griffonia (Bandeiraea) Simplicifolia Lectin I (GSL I, BSL I), Unconjugated
Catalog #:
L-1100-5
Griffonia (Bandeiraea) Simplicifolia Lectin I (GSL I, BSL I), Rhodamine
Catalog #:
RL-1102-2
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