Bis-dPEG®₃-biotin (QBD-10325)

Description

Bis-dPEG®3-biotin, product number QBD-10325, is a novel biotin-labeling product composed of a dPEG®3 spacer with biotin attached to each end of the spacer, Bis-dPEG®3-biotin offers customers unique opportunities for exploiting the biotin-avidin/streptavidin affinity since it can bind to two different avidin/streptavidin binding sites, both intramolecularly and intermolecularly. Indeed, this product was developed specifically to block endogenous biotin found in all cells and tissues so that it did not interfere with targeted biotin-streptavidin interactions in therapeutic and diagnostic applications where endogenous biotin was known to cause problems.

The binding behavior of Bis-dPEG®3-biotin with streptavidin opens up possible uses for the product in the self-assembly of supramolecular constructs and signal amplification. In the first example, if two parts of a potential supramolecular construct both contain avidin or streptavidin, addition of QBD-10325 under empirically determined optimized conditions could trigger self-assembly of the construct as the bis-dPEG®3-biotin binds to the separate avidin or streptavidin molecules in the two halves of the construct. In the second example, multiple streptavidin molecules conjugated to horseradish peroxidase (HRP) could be bound together with a streptavidin-biotinylated protein complex (for example, a biotinylated antibody), thus amplifying the signal from HRP.

Specifications

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References

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.