Azido-PEG4-NHS Ester (CCT-AZ103)


Azido-PEG4-NHS Ester is a labeling reagent that can be used to modify primary amine groups (e.g., a side chain of lysine residues or aminosilane-coated surfaces) with an azido group via a stable amide bond. The azide (N3) group reacts with terminal alkynes via CuAAC, strained cyclooctynes (e.g. DBCO or BCN compounds) via Cu-free click reaction or with phosphine-labeled molecules by a mechanism known as Staudinger chemistry, enabling efficient and specific conjugation of derivatized molecules in biological samples.


Unit Size25 mg, 100 mg, 1000 mg
Molecular weight388.37
Chemical compositionC15H24N4O8
SolubilityDMSO, DMF, DCM, THF, Chloroform
Purity>95% (HPLC)
AppearanceColorless to slightly yellow oil
Storage Conditions-20°C. Desiccate
Shipping ConditionsAmbient temperature

Selected References

  1. Hu, Y., Glazier, R., et al. (2021). DNA-based microparticle tension sensors (µTS) for measuring cell mechanics in non-planar geometries and for high-throughput quantification. Angew Chem Int Ed Engl., Online ahead of print. [PubMed]
  2. Bazrafshan, A. et al. (2021). DNA Gold Nanoparticle Motors Demonstrate Processive Motion with Bursts of Speed Up to 50 nm Per Second. ACS Publications, Online ahead of print. [PubMed]
  3. Bazrafshan, A., et al. (2020). Tunable DNA Origami Motors Translocate Ballistically Over μm Distances at nm/s Speeds. Angew. Chem. Int. Ed.59(24), 9514-21. [PubMed]

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