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Vector Laboratories is closed for the President’s Day on Monday, February 19th. We will be back in the office on Tuesday, February 20th.
We will respond to emails upon our return. Have a wonderful day.
Agarose-GLAGLLAR-Azide is an agarose resin that is pre-activated with azide groups linked to the agarose resin through a short synthetic peptide containing a tryptic cleavage site. The alkyne-modified proteins, or their post-translationally modified forms, are captured from complex protein extracts on the Agarose-GLAGLLAR-Azide resin. Once covalently attached to the resin via copper catalyzed click chemistry, beads can be washed with highest stringency virtually eliminating any non-specifically bound proteins to yield a highly enriched population of nascent molecules. Upon tryptic digestion, this yields a highly pure peptide pool that is ideal for mass spectrometry (e.g., LC MS/MS) based analysis. Importantly, alkyne-modified targeted peptides can be easily identified by MS analysis, providing a confirmation by a mass shift of 172.10 Da
| Unit Size | 2 ml |
|---|---|
| Enrichment target | Alkyne-modified proteins |
| Format | Batch or column purifications |
| Activation level | 5-12 µmol azide groups per mL resin |
| Bead size | Spherical, 50-150 µm |
| Support | 6% Cross-linked agarose |
| Formulation | 50% slurry |
| Preservative | 20% ethanol |
| Appearance | Off-white slurry |
| Storage | 2-8C |
| Shipping Conditions | Ambient temperature |
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