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5(6)-Carboxyrhodamine 110 NHS Ester (FP-1201)

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Description

5(6)-Carboxyrhodamine 110 NHS Ester (also known as Rhodamine Green™ Carboxylic Acid, Succinimidyl Ester, Hydrochloride or 5(6)-CR 110, SE) is the nonsulfonated analog of the Alexa Fluor® 488 dye. The amine-reactive 5(6)-Carboxyrhodamine 110 NHS Ester can be used to create bright and photostable green-fluorescent bioconjugates with excitation/emission maxima ~502/527 nm.

The conjugates of this dye often used for Fluorescence Correlation Spectroscopy (FCS). For many applications, 5(6)-Carboxyrhodamine 110 NHS Ester is preferred over 5-(6)-carboxyfluorescein NHS ester or FITC because of its exceptional photostability and fluorescence insensitivity to pH (4-9).

Specifications

Unit Size5 mg, 25 mg, 100 mg
ReactivityPrimary amine
Abs/Em Maxima502/527 nm
Extinction coefficient76,000 cm-1M-1
SolubilityDMSO, DMF
Spectrally similar dyesAlexa Fluor® 488, DyLight® 488, Fluorescein, Oregon Green 488
Molecular weight471.43
Storage Conditions-20°C.
Shipping ConditionsAmbient temperature

Abs/Em Spectra

MB488

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