In dot blot or western blot application, blocking solutions minimize the non-specific binding of detection antibodies to the membrane that lead to background.
Vector Laboratories offers two solutions formulated to block membranes in blotting applications:
- WestVision™ Block and Diluent — a proprietary, ready-to-use formulation containing Tween 20 that improves signal intensity and resolution without increasing background. It is compatible with both alkaline phosphatase- and peroxidase- based detections systems, and with both chemiluminescence and chromogenic development.
- Animal-Free Blocker™ — a plant-derived universal antibody diluent and blocking reagent for nucleic acid and protein blotting, IHC, and for other applications. It contains no animal-derived protein and can be used as an alternative to sera, BSA, casein, or non-fat dry milk.