Species on Species Detection
When your primary antibody is the same species as your specimen
When a primary antibody is made in the same species as your specimen, the secondary antibody cannot distinguish that primary antibody from endogenous immunoglobulins. This causes high background staining and obscures antigen-specific staining.
Use VECTOR® M.O.M.® (Mouse on Mouse) Kits to significantly reduce endogenous mouse Ig staining when using mouse antibodies on mouse tissue.
- Eliminate tedious calculations and primary antibody pre-binding steps
- See clear, crisp, specific staining
- Introduce two or more labels in a multiple antigen labeling protocol (detect several mouse primary antibodies on the same tissue section)
- Contains proprietary M.O.M. Mouse IgG Blocking Reagent
- Employ either avidin-biotin or polymer technology
- Are compatible with fluorescent or enzyme-based detection
- Are available with or without enzyme or fluorochrome
You can have excellent staining results — for a once difficult application — with the VECTOR M.O.M. Kits.
Explore Species on Species Detection
Mouse on Mouse (M.O.M.®) Kits are specifically designed for detecting and visualizing (unconjugated) mouse primary antibodies in mouse tissue specimens. These kits contain a proprietary reagent that blocks endogenous mouse Ig in specimens therefore preventing non-specific binding of subsequent anti-mouse IgG detection reagents.
Resources
Immunohistochemistry Resource Guide
The Immunohistochemistry Resource Guide will help you navigate through the many choices of detection systems, substrates, and ancillary reagents that complete your workflow.
Immunofluorescence Resource Guide
Producing immunofluorescence (IF) staining results with high specificity and sensitivity begins with choosing the best reagents. This guide will help you:
- Learn about the IF workflow and how to optimize each step
- Quickly identify and choose the most appropriate fluorescent secondary conjugates
- Select the best anti-fade medium to preserve your fluorescence signal for imaging and archiving
Troubleshooting Mouse Primaries on Mouse Tissue
This guide for troubleshooting mouse primary antibodies on mouse tissue describes some common difficulties encountered in this procedure and details strategies for customizing the M.O.M. Kits to address them.