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Species on Species Detection

When your primary antibody is the same species as your specimen

When a primary antibody is made in the same species as your specimen, the secondary antibody cannot distinguish that primary antibody from endogenous immunoglobulins. This causes high background staining and obscures antigen-specific staining.

Use VECTOR® M.O.M.® (Mouse on Mouse) Kits to significantly reduce endogenous mouse Ig staining when using mouse antibodies on mouse tissue.

  • Eliminate tedious calculations and primary antibody pre-binding steps
  • See clear, crisp, specific staining
  • Introduce two or more labels in a multiple antigen labeling protocol (detect several mouse primary antibodies on the same tissue section)

The VECTOR M.O.M. Kits:

  • Contains proprietary M.O.M. Mouse IgG Blocking Reagent
  • Employ either avidin-biotin or polymer technology
  • Are compatible with fluorescent or enzyme-based detection
  • Are available with or without enzyme or fluorochrome

You can have excellent staining results — for a once difficult application — with the VECTOR M.O.M. Kits.

Mouse colon: Smooth muscle actin (m), Mouse on Mouse (M.O.M.) Basic Kit, VECTASTAIN® ABC-AP Kit, VECTOR Red Substrate (red).
Mouse skeletal muscle (frozen, unfixed): Double label - Alpha-sarcoglycan (m), M.O.M. Fluorescein Kit (green). Muscle specific actin (m), M.O.M. Basic Kit, Texas Red® Avidin DCS (red).

Explore Species on Species Detection

Mouse on Mouse (M.O.M.®) Kits are specifically designed for detecting and visualizing (unconjugated) mouse primary antibodies in mouse tissue specimens. These kits contain a proprietary reagent that blocks endogenous mouse Ig in specimens therefore preventing non-specific binding of subsequent anti-mouse IgG detection reagents.


Immunohistochemistry Resource Guide

The Immunohistochemistry Resource Guide will help you navigate through the many choices of detection systems, substrates, and ancillary reagents that complete your workflow.


Immunofluorescence Resource Guide

Producing immunofluorescence (IF) staining results with high specificity and sensitivity begins with choosing the best reagents.  This guide will help you:

  • Learn about the IF workflow and how to optimize each step
  • Quickly identify and choose the most appropriate fluorescent secondary conjugates
  • Select the best anti-fade medium to preserve your fluorescence signal for imaging and archiving


Troubleshooting Mouse Primaries on Mouse Tissue

This guide for troubleshooting mouse primary antibodies on mouse tissue describes some common difficulties encountered in this procedure and details strategies for customizing the M.O.M. Kits to address them.