Elucidate anatomical connections of the nervous system
Neuronal tracers provide an opportunity to visualize neural architecture and identify gap junction coupling in vivo or in whole mounts, slice preparations, or cultured cells. Neuronal Tracers can also be used to detect biotin labels using avidin or streptavidin systems (in conjunction with either chromogenic or fluorescence visualization methods).
Producing immunofluorescence (IF) staining results with high specificity and sensitivity begins with choosing the best reagents. This guide will help you:
- Learn about the IF workflow and how to optimize each step
- Quickly identify and choose the most appropriate fluorescent secondary conjugates
- Select the best anti-fade medium to preserve your fluorescence signal for imaging and archiving