Live Cell Imaging

Fluorescent dyes and antifade reagent for use in live cell imaging

Whereas immunostaining provides a snapshot in time of a cell or tissue, live cell imaging allows you to observe biological processes over time. Our VectaCellâ„¢ reagents enhance your live cell imaging studies that use conventional or super-resolution fluorescence microscopy, immunocytochemistry, in situ hybridization, or neurobiology applications.

VectaCellâ„¢ Trolox Antifade Reagent reduces phototoxicity and photobleaching of reagents to increase cell viability and prolong signal.

Acidic endosomes stained with VectaCell Acridine Orange in MCF-7 cells expressing GFP.
Mitochondria stained with VectaCell Rhodamine 123 (orange) in MVF-7 cells expressing GFP. Nuclei stained with DAPI (blue).

Resources

Immunofluorescence Resource Guide

Producing immunofluorescence (IF) staining results with high specificity and sensitivity begins with choosing the best reagents.  This guide will help you:

  • Learn about the IF workflow and how to optimize each step
  • Quickly identify and choose the most appropriate fluorescent secondary conjugates
  • Select the best anti-fade medium to preserve your fluorescence signal for imaging and archiving

Download