Avidin & Biotinylated Reagents
Vector Laboratories was a pioneer in the field of histological staining. We were the first company to commercialize a Biotin-Avidin (now also Streptavidin) System, a substantial improvement over earlier staining methods. Many biotin molecules can be coupled to a protein, which allows the biotinylated protein to bind more than one molecule of avidin or streptavidin. If biotinylation is performed under gentle conditions, the biological activity of the protein can be preserved.
Both avidin and streptavidin bind biotin irreversibly, and both proteins have four biotin binding sites. These properties make avidin and streptavidin reagents powerful tools in the detection and purification applications of biotinylated macromolecules.
Use the Biotin-Avidin/Streptavidin System for the detection and localization of antigens, glycoconjugates, and nucleic acids in a range of research applications. Choose from among purified avidin and streptavidin proteins, anti-avidin and anti-streptavidin amplification antibodies, biotinylated reagents, and a range of avidin and streptavidin conjugates with different ligands, such as fluorophores and enzymes.
Explore Avidin & Biotinylated Reagents
Biotinylated Protein A isolated from S. aureus, is a 41 kDa protein which binds to the Fc portion of most immunoglobulins. Protein A can also interact with IgA, IgM and subclasses of IgG from different species.
Avidin and Streptavidin are offered conjugated with either alkaline phosphatase (AP) or horseradish peroxidase (HRP) in concentrate or ready-to-use formats. Use these reagents for enzyme-based detection of biotinylated macromolecules including antibodies and nucleic acids.
Select from traditional or contemporary fluorophore conjugates to meet your specific application requirements.
Select Avidin D for detection of biotinylated proteins. Select Avidin DN for detection of biotin-labeled DNA or RNA probes. Select Streptavidin for solid-phase assays.
Producing immunofluorescence (IF) staining results with high specificity and sensitivity begins with choosing the best reagents. This guide will help you:
- Learn about the IF workflow and how to optimize each step
- Quickly identify and choose the most appropriate fluorescent secondary conjugates
- Select the best anti-fade medium to preserve your fluorescence signal for imaging and archiving