Antigen unmasking solutions reveal antigens for optimum immunostaining
Use Vector Labs’ antigen retrieval reagents with formalin-fixed, paraffin-embedded tissue sections. These reagents (in combination with a high-temperature treatment) unmask antigens for optimum immunolabeling.
Vector offers you two formulations, each supplied as 100x concentrated stocks:
Producing immunofluorescence (IF) staining results with high specificity and sensitivity begins with choosing the best reagents. This guide will help you:
- Learn about the IF workflow and how to optimize each step
- Quickly identify and choose the most appropriate fluorescent secondary conjugates
- Select the best anti-fade medium to preserve your fluorescence signal for imaging and archiving
The process of fixing and heating tissue sections in a formalin-fixation, paraffin-embedding procedure often buries epitopes so that they cannot be detected by your primary antibody. Vector Laboratories' Antigen Unmasking Solutions are highly effective at revealing antigens in formalin-fixed, paraffin embedded tissue sections when used in combination with a high temperature treatment procedure. These Antigen Unmasking Solutions are available in two formulations. The citrate-based solution (H-3300) is pH 6.0; the Tris-based solution (H-3301) is pH 9.0. Here we demonstrate pressure cooker technique for antigen retrieval.