Antigen Retrieval

Antigen unmasking solutions reveal antigens for optimum immunostaining

Use Vector Labs’ antigen retrieval reagents with formalin-fixed, paraffin-embedded tissue sections. These reagents (in combination with a high-temperature treatment) unmask antigens for optimum immunolabeling.

Vector offers you two formulations, each supplied as 100x concentrated stocks:

Lymph Node: Without (left panel) and with (right panel) TRIS-based Antigen Unmasking Solution, Cyclin D1 (rm), ImmPRESS® Anti-Rabbit IgG Kit, DAB (brown) substrate. Hematoxylin QS (blue) counterstain.

Resources

Immunohistochemistry Resource Guide

The Immunohistochemistry Resource Guide will help you navigate through the many choices of detection systems, substrates, and ancillary reagents that complete your workflow.
Learn more about this guide >

Immunofluorescence Resource Guide

Producing immunofluorescence (IF) staining results with high specificity and sensitivity begins with choosing the best reagents.  This guide will help you:

  • Learn about the IF workflow and how to optimize each step
  • Quickly identify and choose the most appropriate fluorescent secondary conjugates
  • Select the best anti-fade medium to preserve your fluorescence signal for imaging and archiving

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Antigen Unmasking using Pressure Cooker Technique

The process of fixing and heating tissue sections in a formalin-fixation, paraffin-embedding procedure often buries epitopes so that they cannot be detected by your primary antibody. Vector Laboratories' Antigen Unmasking Solutions are highly effective at revealing antigens in formalin-fixed, paraffin embedded tissue sections when used in combination with a high temperature treatment procedure. These Antigen Unmasking Solutions are available in two formulations. The citrate-based solution (H-3300) is pH 6.0; the Tris-based solution (H-3301) is pH 9.0.  Here we demonstrate pressure cooker technique for antigen retrieval.