Antigen Retrieval
Antigen unmasking solutions reveal antigens for optimum immunostaining
Use our antigen retrieval reagents with formalin-fixed, paraffin-embedded tissue sections. These reagents — in combination with a high-temperature treatment — unmask antigens for optimum immunolabeling.
Vector Laboratories offers you 2 formulations, each supplied as 100x concentrated stocks.
Embrace the future of multiplexing with antibody removal kits
VectaPlex™ Antibody Removal Kit is a ready-to-use kit designed to remove antibodies and other non-covalently bound detection reagents from formalin-fixed paraffin-embedded (FFPE) tissue sections in immunofluorescence or tyramide signal amplification staining workflows.
Lymph Node: Without (left panel) and with (right panel) TRIS-based Antigen Unmasking Solution, Cyclin D1 (rm), ImmPRESS® Anti-Rabbit IgG Kit, DAB (brown) substrate. Hematoxylin QS (blue) counterstain.
Stomach section stained with six mouse primary antibodies (CD20 (Red), CD34 (Yellow), DES (Cyan), AE1/AE3 (Magenta), CD3 (Grey), VIM (Green)) detected with DyLight™ 488 horse anti-mouse IgG and mounted with VECTASHIELD® PLUS with DAPI (Blue). This composite image was generated from six rounds of immunofluorescent staining using the VectaPlex™ Antibody Removal Kit.
Resources
Producing immunofluorescence (IF) staining results with high specificity and sensitivity begins with choosing the best reagents. This guide will help you:
- Learn about the IF workflow and how to optimize each step
- Quickly identify and choose the most appropriate fluorescent secondary conjugates
- Select the best anti-fade medium to preserve your fluorescence signal for imaging and archiving
