The Superior Antifade - VECTASHIELD Mounting Media VECTASHIELD® Antifade Mounting Medium is a unique, stable formula for preserving fluorescence. VECTASHIELD® Mounting Medium prevents rapid photobleaching of fluorescent proteins and fluorescent dyes. Features: Inhibits photobleaching of dyes and fluorescent proteins Ideal refractive index Ready to use No warming necessary Offered with nuclear or cytoskeletal counterstains Available in non-hardening or hardening formulations Can be stored without sealing for long term analysis Easy to use The original VECTASHIELD® Mounting Medium does not solidify, but remains a liquid on the slide and can be stored without sealing. If desired, coverslips can be sealed around the perimeter with nail polish or a plastic sealant. Mounted slides should be stored at 4 °C, protected from light. VECTASHIELD® HardSet™ Mounting Medium preserves fluorescence and hardens after coverslipping. After approximately 15 minutes at room temperature, the coverslip will become immobilized, and optimal antifade ability and refractive index will be achieved. VECTASHIELD® Antifade Mounting Media Formulations For more information on each VECTASHIELD® formulation, please click on the links below: VECTASHIELD® Antifade Mounting Medium (H-1000, non-hardening) VECTASHIELD® Antifade Mounting Medium with DAPI (H-1200, non-hardening) VECTASHIELD® Antifade Mounting Medium with PI (H-1300, non-hardening) VECTASHIELD® Antifade HardSet™Mounting Medium (H-1400, hardening) VECTASHIELD® Antifade HardSet™Mounting Medium with DAPI (H-1500, hardening) VECTASHIELD® Antifade HardSet™Mounting Medium with TRITC-Phalloidin (H-1600, hardening) VECTASHIELD® Mounting Medium in Super Resolution Microscopy The optimal medium for super-resolution imaging methods maximizes the lifetime and photo-switching characteristics of fluorophores. VECTASHIELD® Mounting Medium has been shown to be compatible with a number of fluorophores used in super resolution methods including STORM, STED, and 3D-SIMM imaging, such as Cy5 or Alexa fluor 647, resulting in greater convenience and reproducibility of the method. (Olivier, N. et al., 2013, Biomedical Optics Express, Vol. 4 No. 6, pp. 885-899). VECTASHIELD® Mounting Medium Antifade Comparison Other manufacturers measure the antifade properties of their mountants using labeled microspheres or arrayed spots. Vector Labs prefers to measure antifade properties of VECTASHIELD® mountants using frozen tissue sections immunohistochemically stained with fluorescently labeled secondary antibodies. Antifade capability is measured using a 40x objective with real time imaging over 30 seconds of continuous exposure to the excitation illumination. Individual intensity measurements are recorded from 6 separate labeled regions and the average is calculated. The intensity after 30 second exposure is expressed as a percentage of the intensity at zero time. The values for PG are taken from the manufacturer s published results.