A Brilliant Solution for Fluorescence Signal Retention

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Introducing VECTASHIELD® Vibrance™ Antifade Mounting Media

 

VECTASHIELD® Vibrance™ products are completely new formulations of curing antifade mounting media for immunofluorescence applications. These newest additions to our VECTASHIELD portfolio were developed with customer feedback to improve on parameters such as ease of use and retention of specific staining intensity over time. VECTASHIELD® Vibrance™ Antifade Mounting Media are new tools to help investigators see more and do more with each experiment. 

Why VECTASHIELD® Vibrance™ Mounting Media?

  • Superior antifade/anti-photobleaching properties across the spectrum
  • Compatibility with commonly used fluorophores
  • View sections one hour after mounting
  • No tone or autofluorescent background, even after curing
  • Room temperature storage of slides with extended archiving time
  • Minimal bubble formation, even after several weeks storage
  • Curing formulations with choice of counterstain (DAPI) or no counterstain
  • 12 month product expiration date
Plus the same features you have come to expect of VECTASHIELD® brand:
  • Ideal refractive index
  • Easy to use
  • Ready to use, requires no warming
  • No sealing of coverslips required (curing formulations)

Easy Application and Use of VECTASHIELD® Vibrance™

Excellent Antifade Properties Across the Spectrum, with Commonly Used Fluorophores

VECTASHIELD® Vibrance™ Mounting Media provide excellent protection against fading across the visible spectrum, even under far-red wavelengths. They are compatible with most commercially available fluorophores.

Excellent antifade properties of VECTASHIELD® Vibrance™ Mounting Media

Viewable in as Little as One Hour After Mounting – No Need to Wait 24 Hours

Competitor immunofluorescence workflows recommend slides be viewed 24 hours after mounting. VECTASHIELD® Vibrance™ Mounting Media, however, allow same-day viewing to accelerate discovery without sacrificing signal intensity or retention.

Improved retention of fluorescence with VECTASHIELD® Vibrance™ Mounting Medium. Serial sections of human colon tissue (FFPE) stained for cytokeratin (mouse primary antibody AE1/AE3) and detected with fluorescein-conjugated horse anti-mouse IgG secondary antibody (FI-2000). One hour after mounting with either VECTASHIELD® Vibrance™ (top row) or a competitor mounting medium (bottom row), the sections were imaged at the intervals indicated. Note the improved retention of fluorescent signal for sections mounted with VECTASHIELD® Vibrance™ Mounting Medium.

Extended Archiving of Mounted Sections at Room Temperature

Archiving fluorescently stained sections at room temperature often leads to signal reduction and dessication of the specimen. Slides mounted with VECTASHIELD® Vibrance™ Medium, however, can be stored for weeks at ambient temperature, in standard slide boxes, with no loss of specific signal intensity or specimen integrity. The stabilizing effect of VECTASHIELD® Vibrance™ Mounting Medium provides greater flexibility for imaging and storing fluorescently stained samples.

Serial sections of human colon tissue (FFPE) mounted with either VECTASHIELD® Vibrance™ Mounting Medium with DAPI (H-1800, left image) or competitor mounting medium with DAPI (right image), each stored at room temperature for two weeks and then imaged.

Note absence of bubbles and background in blue spectrum in left image using VECTASHIELD® Vibrance™ Mounting Medium with DAPI compared with right image, after two weeks at room temperature.

…with No Specimen Obscuring Retraction or Bubble Formation

Another problem commonly observed in immunoflourescence staining is the formation over time of bubbles under the coverslip, or shrinking (retraction) of the media. This phenomenon detracts from the overall quality of the sections and can obscure specific staining. The unique formulation of VECTASHIELD® Vibrance™ Mounting Medium provides a uniform sheet of medium under the coverslip, with essentially no bubble formation or retraction—even after many weeks of storage at room temperature.

Serial sections of human colon tissue (FFPE) stained for cytokeratin (AE1/AE3, mouse primary) followed by Alexa Fluor™ 488 anti-mouse IgG secondary antibody. Sections were mounted with either VECTASHIELD® Vibrance™ Mounting Medium (H-1700, left image) or competitor mounting medium (right image) stored at room temperature for two weeks, and then imaged.

Note absence of bubbles and background in green spectrum in left image using VECTASHIELD® Vibrance™ Mounting Medium compared with right image, after two weeks at room temperature.

Customer Testimonials

Ordering Information

Product Unit Size No Counterstain With DAPI Counterstain With PI Counterstain TRITC-Phalloidin

VECTASHIELD®Vibrance™ Antifade Mounting Medium (hardening)

2ml, 10ml H-1700 H-1800

VECTASHIELD® Antifade Mounting Medium (non-hardening)

10ml H-1000 H-1200 H-1300

VECTASHIELD®HardSet™ Antifade Mounting Medium (hardening)

10ml H-1400 H-1500 H-1600