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Vector Fusion-Aid HA Kit, (Agarose Anti-HA)

Catalog Number: MB-0734

Availability: In stock

$240.00
  • Buy 10 for $216.00 each and save 10%
  • Buy 30 for $192.00 each and save 20%
Unit Size: 1 kit

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Overview

    Features:

    The Fusion-Aid™ Kits are designed to immunoprecipitate/isolate fusion proteins containing the appropriate protein tag. The Fusion-Aid™ Kit has affinity-purified primary antibodies coupled directly to agarose beads. In contrast to traditional methods, this eliminates the contamination of the isolated proteins with coprecipitating primary antibodies. The spin columns provided with the kit also facilitate rapid immunoprecipitation and maximum protein recovery.

     

    Kit components:

    • 0.5ml agarose beads coupled with affinity purified antibody agains Human influenza hemagglutinin (HA)
    • 5 spin columns
    • 5 collection tubes 

    Additional Information

    Catalog Number MB-0734
    Unit Size 1 kit
    Country of Manufacture United States
    Applications Affinity Chromatography
    Matrix Conjugate Fusion Protein Tag
    Fusion Protein Tag HA (Human Influenza Virus Hemagglutinin)
Additional Info

    Details

    Creating fusion proteins using recombinant DNA techniques is the preferred method to purify or detect many proteins of interest.

    Two proteins that are commonly fused to proteins of interest are green fluorescent protein (GFP) and maltose binding protein (MBP). Alternatively, proteins of interest are fused to a specific peptide sequence rather than an entire protein. This peptide sequence defines an epitope that can be readily detected with a specific antibody. Two commonly used peptide tags are derived from human influenza virus hemagglutinin (HA) and c-Myc.

    Fusion Protein Purification. The Fusion-Aid Kits are designed to immunoprecipitate/isolate fusion proteins containing the appropriate protein tag. The Fusion-Aid Kit has affinity-purified primary antibodies coupled directly to agarose beads. In contrast to traditional methods, this eliminates the contamination of the isolated proteins with coprecipitating primary antibodies. The spin columns provided with the kit also facilitate rapid immunoprecipitation and maximum protein recovery.
    Vector Fusion-Aid Kits contain the following:

    • 0.5 ml agarose beads coupled with affinity purified antibody to the specific tag
    • 5 spin columns
    • 5 collection tubes
    • a detailed protocol
    lane 1: M.W. Marker. lane 2: Crude lysate. lane 3: Eluate from Fusion-Aid HA kit resin. lane 4: Eluate using traditional method (polyclonal goat anti-HA was added to crude lysate, applied to agarose protein A and eluted).
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Protocols/Data Sheet/SDS

    Protocols/Data Sheet/SDS

    SDS Protocol Data Sheet