|Unit Size||1 kit|
|Country of Manufacture||United States|
|Applications||Blotting Applications, Elispot|
|Technology||ABC (Streptavidin/Biotin) Systems|
|Detection Enzyme(s)||Alkaline Phosphatase|
|Target Species||No antibody included|
Greater sensitivity can be achieved by using an amplified detection procedure. Our biotin/avidin and biotin/streptavidin systems introduce a large number of enzymes to the target site providing signal amplification while maintaining low background.
The VECTASTAIN ABC-AmP is an amplified ABC alkaline phosphatase reagent for the detection of mouse or rabbit primary antibodies on nitrocellulose or PVDF membranes. The signal can be visualized using either a chemiluminescent/ chemifluorescent or a chromogenic substrate. Used with the DuoLuX Chemiluminescent / Chemifluorescent Substrate, the VECTASTAIN ABC- AmP system produces a very high and sustained light emission signal, with low background, and permanent fluorescence.
The sensitivity of VECTASTAIN ABC-AmP Reagent combined with the DuoLux substrate allows for the detection of as little as 1 pg of target protein.
The VECTASTAIN ABC-AmP Reagent is sufficient to develop approximately twenty 100 cm2blots.
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