VECTASTAIN® ABC-AmP Kit, for Rabbit IgG, Chromogenic Western Blot Detection

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Cat. No: AK-6401
Price: $322.00
Unit Size: 1 kit
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  • Amplified ABC alkaline phosphatase reagent for protein blot detection
  • Uses biotin/streptavidin technology
  • Contains highly sensitive chromogenic substrate, BCIP/NBT (indigo color)
  • Sufficient reagents to develop twenty 100 cm2 blots

Kit Contents:

  • 250 ml 10x Casein Solution
  • 0.25 ml Biotinylated Anti Rabbit IgG
  • 0.5 ml Reagent A and 0.5 ml Reagent B for VECTASTAIN ABC-AmP Reagent
  • BCIP/NBT Substrate Kit (produces 200 ml of substrate solution)
Cat. No. AK-6401
Unit Size 1 kit
Country of Manufacture United States
Applications Blotting Applications, Elispot
Technology ABC (Streptavidin/Biotin) Systems
Detection Enzyme(s) Alkaline Phosphatase
Target Species Rabbit
Format Concentrate

More Information

Greater sensitivity can be achieved by using an amplified detection procedure. Our biotin/avidin and biotin/streptavidin systems introduce a large number of enzymes to the target site providing signal amplification while maintaining low background.

The VECTASTAIN ABC-AmP is an amplified ABC alkaline phosphatase reagent for the detection of mouse or rabbit primary antibodies on nitrocellulose or PVDF membranes. The signal can be visualized using either a chemiluminescent/ chemifluorescent or a chromogenic substrate. Used with the DuoLuX® Chemiluminescent/Chemifluorescent Substrate, the VECTASTAIN ABC- AmP system produces a very high and sustained light emission signal, with low background, and permanent fluorescence.

The sensitivity of VECTASTAIN ABC-AmP Reagent combined with the DuoLux substrate allows for the detection of as little as 1 pg of target protein.

Each VECTASTAIN ABC-AmP kit provides sufficient reagents to develop approximately twenty 100 cm2 blots.

Kit  Contents:

  • VECTASTAIN ABC-AmP reagent
  • 10x casein solution
  • biotinylated anti-rabbit IgG secondary antibody
  • BCIP/NBT chromogenic substrate

Reagents contained in the kits can also be purchased separately.

Safety Information

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