|Unit Size||1 kit|
|Country of Manufacture||United States|
|Applications||Blotting Applications, Elispot|
|Technology||ABC (Streptavidin/Biotin) Systems|
|Detection Enzyme(s)||Alkaline Phosphatase|
Greater sensitivity can be achieved by using an amplified detection procedure. Our biotin/avidin and biotin/streptavidin systems introduce a large number of enzymes to the target site providing signal amplification while maintaining low background.
The VECTASTAIN® ABC-AmP is an amplified ABC alkaline phosphatase reagent for the detection of mouse or rabbit primary antibodies on nitrocellulose or PVDF membranes. The signal can be visualized using either a chemiluminescent/ chemifluorescent or a chromogenic substrate. Used with the DuoLuX™ Chemiluminescent/Chemifluorescent Substrate, the VECTASTAIN® ABC- AmP system produces a very high and sustained light emission signal, with low background, and permanent fluorescence.
The sensitivity of VECTASTAIN® ABC-AmP Reagent combined with the DuoLux substrate allows for the detection of as little as 1 pg of target protein.
Each VECTASTAIN® ABC-AmP kit provides sufficient reagents to develop approximately twenty 100 cm2 blots.
Reagents contained in the kits can also be purchased separately.