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VECTASTAIN ABC-AmP Kit, for Rabbit IgG, Chemiluminescent Western Blot Detection

Catalog Number: AK-6601

Availability: In stock

$365.00
  • Buy 10 for $328.50 each and save 10%
  • Buy 30 for $292.00 each and save 20%
Unit Size: 1 kit

Protein detection using VECTASTAIN ABC-AmP Reagent and DuoLuX Substrate Zoom

Overview

    Features:

    • Amplified ABC alkaline phosphatase reagent for protein blot detection
    • Uses biotin/streptavidin technology
    • Use with DuoLuX™ Chemiluminescent/Chemifluorescent Substrate to produce a very high and sustained light emissions signal, with low background, and permanent fluorescence
    • Can detect as little as 1 pg target protein with DuoLuX™ Substrate
    • Sufficient reagents to develop twenty 100 cm2 blots

    Kit Contents:

    • 250 ml 10x Casein Solution
    • 0.25 ml Anti-Rabbit IgG
    • 0.5 ml Reagent A and 0.5 ml Reagent B for VECTASTAIN® ABC-AmP Reagent
    • 100 ml DuoLuX™ Chemiluminescent Substrate

    Additional Information

    Catalog Number AK-6601
    Unit Size 1 kit
    Country of Manufacture United States
    Applications Blotting Applications, Elispot
    Technology ABC (Streptavidin/Biotin) Systems
    Detection Enzyme Alkaline Phosphatase
    Target Species Rabbit
    Format Concentrate
Additional Info

    Details

    Greater sensitivity can be achieved by using an amplified detection procedure. Our biotin/avidin and biotin/streptavidin systems introduce a large number of enzymes to the target site providing signal amplification while maintaining low background.

    The VECTASTAIN® ABC-AmP is an amplified ABC alkaline phosphatase reagent for the detection of mouse or rabbit primary antibodies on nitrocellulose or PVDF membranes. The signal can be visualized using either a chemiluminescent/ chemifluorescent or a chromogenic substrate. Used with the DuoLuX™ Chemiluminescent/Chemifluorescent Substrate, the VECTASTAIN® ABC- AmP system produces a very high and sustained light emission signal, with low background, and permanent fluorescence.

    The sensitivity of VECTASTAIN® ABC-AmP Reagent combined with the DuoLuX™ substrate allows for the detection of as little as 1 pg of target protein.

    Each VECTASTAIN® ABC-AmP kit provides sufficient reagents to develop approximately twenty 100 cm2 blots.

    Kit Contents:

    • VECTASTAIN® ABC-AmP reagent
    • 10x casein solution
    • biotinylated anti-rabbit IgG secondary antibody
    • DuoLuX™ Chemiluminescent/ Fluorescent Substrate.
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