VECTASTAIN ABC-AmP Kit, for Rabbit IgG, Chemiluminescent Western Blot Detection

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Cat. No: AK-6601
Price: $398.00
Unit Size: 1 kit

Overview

Features:

  • Amplified ABC alkaline phosphatase reagent for protein blot detection
  • Uses biotin/streptavidin technology
  • Use with DuoLuX™ Chemiluminescent/Chemifluorescent Substrate to produce a very high and sustained light emissions signal, with low background, and permanent fluorescence
  • Can detect as little as 1 pg target protein with DuoLuX™ Substrate
  • Sufficient reagents to develop twenty 100 cm2 blots

Kit Contents:

  • 250 ml 10x Casein Solution
  • 0.25 ml Anti-Rabbit IgG
  • 0.5 ml Reagent A and 0.5 ml Reagent B for VECTASTAIN® ABC-AmP Reagent
  • 100 ml DuoLuX™ Chemiluminescent Substrate
Cat. No. AK-6601
Unit Size 1 kit
Country of Manufacture United States
Applications Blotting Applications, Elispot
Technology ABC (Streptavidin/Biotin) Systems
Detection Enzyme(s) Alkaline Phosphatase
Conjugate N/A
Color of Fluorescence N/A
Target Species Rabbit
Format Concentrate
Blocking Action N/A

More Information

Greater sensitivity can be achieved by using an amplified detection procedure. Our biotin/avidin and biotin/streptavidin systems introduce a large number of enzymes to the target site providing signal amplification while maintaining low background.

The VECTASTAIN® ABC-AmP is an amplified ABC alkaline phosphatase reagent for the detection of mouse or rabbit primary antibodies on nitrocellulose or PVDF membranes. The signal can be visualized using either a chemiluminescent/ chemifluorescent or a chromogenic substrate. Used with the DuoLuX™ Chemiluminescent/Chemifluorescent Substrate, the VECTASTAIN® ABC- AmP system produces a very high and sustained light emission signal, with low background, and permanent fluorescence.

The sensitivity of VECTASTAIN® ABC-AmP Reagent combined with the DuoLuX™ substrate allows for the detection of as little as 1 pg of target protein.

Each VECTASTAIN® ABC-AmP kit provides sufficient reagents to develop approximately twenty 100 cm2 blots.

Kit Contents:

  • VECTASTAIN® ABC-AmP reagent
  • 10x casein solution
  • biotinylated anti-rabbit IgG secondary antibody
  • DuoLuX™ Chemiluminescent/ Fluorescent Substrate.

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